Swetha Balakrishnan

6 Questions 9 Answers 0 Followers

Questions related from Swetha Balakrishnan

I am working on ISSR primers. I get bands for some samples and a smear in others. Why?

Hi, I am currently working on ISSR primers for my algal samples. When I did PCR for my samples along with some marine algal specimens, I could observe bands for the marine specimens and a smear...

16 May 2018 950 3 View

Which is a very successful procedure for extraction of genomic DNA from herbarium sample?

Greeting to all researchers! Can anyone suggest me with a successful procedure for extraction of genomic DNA from herbarium material of Red algae (Rhodophyceae). So far we are successful in...

08 March 2017 7,787 5 View

Is there any expected range of GC content variation for identificattion of sub species?

I have done rbcL sequencing of two populations of the same species of algae. On calculating the GC content of the sequenced product I got a 1% difference between the two populations. Does this...

22 February 2017 4,903 2 View

Does chloroform melt ependorff or other larger centrifugr tubes?

Hi all! While doing my DNA isolation I encountered a new problem. After the addition of chloroform and during centrifugation, the ependorff and 50mL centrifuge tubes melted. Can anyone provide me...

09 January 2017 2,767 12 View

What is the significance of ITS and ISSR region?

I would like to do sequencing of a species of Charophyceae, for which, I have collected a few morphologically differing members from different locations. I would like determine if there is any...

08 October 2016 8,070 5 View

If no bands are seen does that mean that genomic DNA has not been isolated?

I have don't DNA isolation using CTAB method and got good precipitation in the isopropanol stage. But finally when I analyzed my samples in 0.8% agarose gel I could not find any resolved DNA...

10 April 2016 3,509 3 View