Can we precipitate RNA alone but leave DNA in aqueous solution?

Assuming I would like to use some chemical mediated process of precipitating RNA and polysacharides (from tissue lysate) but leave genomic DNA intact in the aqueous layer. What are the best...

06 July 2018 2,757 3 View

Can we store taq polymerase, respective buffer, primers, probes together as single constitution?

Many commercial diagnostic kits send single reagent (Premix) to which the user must just add respective DNA and perform Real Time PCR. What are the key parameters to be considered while preparing...

05 June 2018 5,717 7 View

What could be the reason for no signal at all in Taqman FAM-BHQ1 probe and HEX-BHQ1 probe?

I have two targets A and B both are known sequences cloned into plasmids, The primers Tm for A is 54 and respective probe Tm is 63 The primers Tm for B is 59 and respective probe Tm is 69. PCR...

05 June 2018 6,152 7 View

What if the distance between Sense primer and Sense probe is more than 90 bp?

We have an issue with the Taqman Probe (FAM-BHQ1) based real time detection of target. What we have been observing is at higher copy numbers >2Crore we are getting very good amplification plots...

03 April 2018 7,143 6 View

Which is the best inhouse protocol for midi and maxi prep plasmid DNA isolation?

Looking for midi and maxi plasmid isolation protocols from E coli (high copy number plasmid) cultures above 100ml (LB) Protocols tried so far: Sambrook's midi and maxi prep Alkaline lysis...

03 April 2018 183 4 View

What can improve copy number detection sensitivity of real time pcr FAM-BHQ1 probe based?

I am working with development of viral diagnostic kits using Real Time PCR (Probe based) detection. As per standard procedures we do use plasmid carrying viral gene as control and to determine the...

10 November 2017 2,666 22 View

How to calculate copy number for a plasmid and make necessary dilutions. Thermoscientific tool?

How can one calculate the copy number with following details: plasmid size 4000 bp concentration 1600 ng/ul Now I did try using thermoscientific online tool which does tell u total copy numbers...

07 August 2017 5,549 1 View

What are the strategies to design a 1 Kb in house DNA ladder?

I am looking for strategies which most of the commercial companies use for production of 1 Kb DNA ladder. I did come across good journal for a 100 bp ladder where they have designed primers using...

07 August 2017 7,624 4 View

How to asses the efficiency of bisulfite treatment of genomic DNA?

To perform a methylation specific PCR one has to treat the DNA with bisulfite. But are there any standard measurable ways to determine to how much extent the conversion of unmethylated Cytosine to...

11 December 2016 3,901 2 View

Determine hypo and hypermethylation state using Real Time PCR?

11 December 2016 7,757 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View