I need to screen the toxicity of several compounds that will be applied locally to an open wound of bone and muscle injury, which human cell lines (good for several passages) would be most appropriate for this experiment beside hMSC?
Thanks Dr. Rahman. I am looking for a non-cancerous cell lines, and either immortalized or good for a least a dozen doublings or passages. My understanding is that with the declining proliferation of HSMM, it may not be suitable for this type of high throughput screening.
for high throughput screening, how about "Establishing cell line" using bone marrow-derived mesenchymal stem cells since they possess both myogenic and osteoblast potential?