Hi,

It's depend on you bacteria, i can give you an exemple for Geobacter metallireducens:

Anaerobic Citrate Medium

Composition per liter:

Ferric citrate................................................................................ 17.0g

Sodium acetate.............................................................................. 6.8g

NaHCO3 ........................................................................................ 2.5g

NH4Cl ........................................................................................... 1.5g

NaH2PO4·H2O............................................................................... 0.6g

KCl................................................................................................ 0.1g

Trace elements solution ...........................................................10.0mL

Wolfe’s vitamin solution ..........................................................10.0mL

pH 7.0 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

Na2WO4 ................................................................................... 25.0mg

NiCl2·6H2O.............................................................................. 24.0mg

Wolfe’s mineral solution ...............................................................1.0L

Wolfe’s Mineral Solution:

Composition per liter:

MgSO4·7H2O................................................................................ 3.0g

Nitrilotriacetic acid ....................................................................... 1.5g

NaCl .............................................................................................. 1.0g

MnSO4·H2O.................................................................................. 0.5g

CaCl2............................................................................................. 0.1g

CoCl2·6H2O .................................................................................. 0.1g

FeSO4·7H2O.................................................................................. 0.1g

ZnSO4·7H2O ................................................................................. 0.1g

AlK(SO4)2·12H2O....................................................................... 0.01g

CuSO4·5H2O............................................................................... 0.01g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·2H2O .......................................................................... 0.01g

Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic

acid to approximately 500.0mL of water and adjust to pH 6.5 with

KOH to dissolve the compound. Bring volume to 1.0L with remaining

water and add remaining compounds one at a time.

Preparation of Trace Elements Solution: Combine components.

Mix thoroughly.

Wolfe’s Vitamin Solution:

Composition per liter:

Pyridoxine·HCl ........................................................................10.0mg

p-Aminobenzoic acid.................................................................5.0mg

Lipoic acid ................................................................................. 5.0mg

Nicotinic acid............................................................................. 5.0mg

Riboflavin .................................................................................. 5.0mg

Thiamine·HCl ............................................................................ 5.0mg

Calcium DL-pantothenate........................................................... 5.0mg

Biotin ......................................................................................... 2.0mg

Folic acid ................................................................................... 2.0mg

Vitamin B12 ................................................................................ 0.1mg

Preparation of Wolfe’s Vitamin Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Prepare and dispense medium under 80%

N2 + 20% CO2. Add ferric citrate to 500.0mL of boiling distilled/deionized

water. Mix thoroughly. Cool to room temperature while sparging

with 80% N2 + 20% CO2. Add remaining components. Add

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Adjust pH to 7.0 with NaOH. Continue sparging with 80% N2 + 20%

CO2. Anaerobically distribute into tubes. Autoclave for 15 min at 15

psi pressure–121°C.

I hope this can help you,

Good luck.

Amina Cherchali Actually it is a mixed culture of anaerobic bacteria and not a pure culture The mixed culture has the following bacteria:-

(Shewanella putrefaciens IR-1, Clostridium butyricum, Desulfuromonas acetoxidans, Geobacter metallireducens, Geobacter sulfurreducens, Rhodoferax ferrireducens, Aeromonas hydrophilia (A3), Pseudomonas aeruginosa, Desulfobulbus propionicus, Geopsychrobacter electrodiphilus, Geothrix fermentans, Shewanella oneidensis DSP10, Escherichia coli, Rhodopseudomonas palustris, Ochrobactrum anthropic YZ-1, Desulfovibrio desulfuricans, Acidiphilium sp.3.2Sup5, Klebsiella pneumoniae L17, Thermincola sp.strain JR, Pichia anomala.)

So, I want to know the commonly used growth medium for these mixed anaerobic bacteria.

doi.org/10.1371/journal.pone.0113379

doi:10.3390/ijerph17031121