I'm getting the formate peak around 8.3 ppm. But the main problem is the quantification of the product.
If you could give me some instructions on quantifying the product from the proton NMR, that would be very helpful.
Dear Sumit,
admittedly I am not even sure whether there is a product in your spectra or just some usual contaminants .... anyways:
Short Protocol:
1) For quantification you have to spike a internal reference with a defined molar concentration.
2) you integrate a NMR signal of your reference and a Signal of your product
3) you divide both integrals by the number of hydrogens inducing this signals (e.g. divide by 3 for a methyl group).
4) The ratio of the divided integals is the molar ratio of Product/Reference. If you know the molar conc. of the reference you know the molar conc. of the product
Comments:
- NMR is simple for quantification be cause this method does NOT need compound specific absorption (like UV or similar). The NMR response is for all compounds the same!
- This works if the magnetisation of both compounds (Product AND reference) is fully relaxed. This has to be ensured by long interscan delay (e.g. 30 or 60s) or small flip-angles of the pulse (e.g. 30deg).
- There are also methods with external standard which are helpful if the instrument was "calibrated".
I hope this does help
Alfred
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