I'm interested in culturing bladder cancer spheroids in matrigel, and I'm not sure about which conditions would be more appropriate (medium and growth factors). I've found oine paper (Lee et al, Cell 2018) where they use hepatocyte medium and EGF, and another one (Mullenders, PNAS 2019) where they add FGF10, FGF7 and FGF2 to DMEM/F12. I'd appreciate any suggestions, thanks!

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