How to integrate OpenComet in Micromanager?

Hello! Has anyone managed to integrate opencomet in micromanager software?? I am installing opencomet.jar at mmplugins folder but it does not appear anywhere in the program. thank you!!

25 March 2020 2,036 3 View

What is the best probe height to measure Caspase-3 with a fluorescence kit using Synergy H1 microplate reader?

Hi, I'm measuring cellular Ca-3 activity with a fluorescence kit (EnzChek Caspase-3 Assay Kit 1) using a Synergy H1 microplate reader. With this current fluorescent kit the final volume to...

01 May 2018 8,793 4 View

How can I draw the spectral and spatial beampattern of a signal?

I have a wideband signal represented in a matrix, such that the number of rows corresponds to the number of frequency bins (N), and the number of columns corresponds to the number of antennas...

04 February 2018 1,900 5 View

Which HA-derived molecules are better to activate CD44?

Hi, I'm looking for HA or HA-derived oligosaccharides in order to provide a positive stimulous and activate CD44, for in vitro migration/invasion experiments. I was wondering if anyone has...

06 October 2016 8,114 1 View

How to create the confidence intervals of a PGLS in R?

11 July 2016 5,220 4 View

How can I coat an IrO2 on Ti substrate electrode with liquid Nafion dispersion?

Hi all, Currently I'm trying to coat the IrO2 electrode, which acts as anode, with a liquid Nafion Dispersion using a dipper. However, this coating does not work as chlorine is produced after...

28 March 2016 174 8 View

Can anyone provide some information about MOF?

We are interested in trying some of our chemistry to make MOF, but have no idea about how to work. I would need some info in how to characterise basically the main basic things.

27 January 2015 6,553 2 View

Does anyone know a reference for the characterization of graphene?

Physico-chemical characterization.

30 November 2013 3,085 4 View

Problems with Microsatellite amplification from fish eggs

We are having problems in PCR amplification of DNA extracted from fish eggs (tuna). DNA extracted from muscle tissue using the same methodolgy works fine. Any help would be really appreciated.

12 November 2013 8,714 8 View

Is it important to add HEPES to primary cell cultures for proliferation assays?

Is there any difference in HEPES requirement when working with mice or human cells?

10 December 2012 3,699 12 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View