What is the best probe height to measure Caspase-3 with a fluorescence kit using Synergy H1 microplate reader?

Hi, I'm measuring cellular Ca-3 activity with a fluorescence kit (EnzChek Caspase-3 Assay Kit 1) using a Synergy H1 microplate reader. With this current fluorescent kit the final volume to...

01 May 2018 8,793 4 View

How can I draw the spectral and spatial beampattern of a signal?

I have a wideband signal represented in a matrix, such that the number of rows corresponds to the number of frequency bins (N), and the number of columns corresponds to the number of antennas...

04 February 2018 1,900 5 View

Which HA-derived molecules are better to activate CD44?

Hi, I'm looking for HA or HA-derived oligosaccharides in order to provide a positive stimulous and activate CD44, for in vitro migration/invasion experiments. I was wondering if anyone has...

06 October 2016 8,114 1 View

How to create the confidence intervals of a PGLS in R?

11 July 2016 5,220 4 View

How can I coat an IrO2 on Ti substrate electrode with liquid Nafion dispersion?

Hi all, Currently I'm trying to coat the IrO2 electrode, which acts as anode, with a liquid Nafion Dispersion using a dipper. However, this coating does not work as chlorine is produced after...

28 March 2016 174 8 View

Can anyone provide some information about MOF?

We are interested in trying some of our chemistry to make MOF, but have no idea about how to work. I would need some info in how to characterise basically the main basic things.

27 January 2015 6,553 2 View

Does anyone know a reference for the characterization of graphene?

Physico-chemical characterization.

30 November 2013 3,085 4 View

Problems with Microsatellite amplification from fish eggs

We are having problems in PCR amplification of DNA extracted from fish eggs (tuna). DNA extracted from muscle tissue using the same methodolgy works fine. Any help would be really appreciated.

12 November 2013 8,714 8 View

Is it important to add HEPES to primary cell cultures for proliferation assays?

Is there any difference in HEPES requirement when working with mice or human cells?

10 December 2012 3,699 12 View

Spleenocytes proliferation and cell death.

When doinf CFSE-proliferation assay with spleen cells from mice I get more then 60% of cell death after 3 days, specially in non-stimulated wells. Same protocol works perfect with PBMCs from...

06 May 2012 3,616 9 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View

Simulation or Experiment? How do we choose?

If we want to assess if a phenomenon is going in a direction we expect, we can simulate it, using digital models, possibly agent models factoring in known properties and interaction features. We...

12 February 2021 1,141 21 View

Vth increase with programming SONOS Flash in Tcad?

Hello, I've been working on this issue for more than a month now.... I made a basic SONOS flash structure using TCAD Sentaurus sProcess. (tunnel oxide/nitride/blocking oxide : 4/6/6 [nm]) Then,...

11 February 2021 3,337 3 View

How to identify when a gaussian program is about terminate normally?

Dear all, I am trying to optimize a bimetallic complex using # opt=tight b3lyp/lanl2dz scf=qc geom=connectivity. The program is running for around 5 days but the size of the .chk remains same...

05 February 2021 8,721 3 View