I need some golgi markers for co-immunofluorescence. In particular, I'd like to see if my gene co-localizes with cis-golgi and TGN (Trans-Golgi network). Would GalNacT2 and TGN38 or 46 be ok for this? What's the difference between TGN38 and 46? Does GalnacT2 only stain cis-golgi?
Thank you for your help!
For human Golgi Zone in general I have a very good experience with mouse anti-human Golgi Zone (# MAB1271, Millipore).
GalnacT2 is a trans-golgi marker and there are several antibodies available from Santa Cruz Biotech.
Anti GM130. It's been tested in 3D cultures as well and stains (IF) right at the apical surface (pointing to the luminal space) of epithelial cells comprising the acinar structure.
Thank you for your answer guys! Actually, since I'm going to do ImmunoFluorescence on Xenopus ectodermal explants, I will have to overexpress one of these markers, because generally anti-human or mouse antibodies do not work very well on Xenopus. Do you think I could use GFP-tagged forms of GM130 and TGN38?
We had made a protein specific monoclonal antibody to rat liver beta1-4 galactosyltransferase before (J Histochem Cytochem 42(3):363, 1994). However, I am not sure whether this antibody works on Xenopus tissues or not.
TGN46 is the human homologue of rat TGN38. They reside in the membrane of the trans-Golgi network (TGN) and also cycle to the plasma membrane, but only a small percentage is outside the TGN, usually a few vesiclular structures per cell.
hi guys! have you tested the gm130 of BD, on mouse cells as well?? I have problems in staining neuronal cells derived from mouse.thanks
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