Gabriele Colozza

6 Questions 18 Answers 0 Followers

Questions related from Gabriele Colozza

Bleed through 680-800 IRDye LiCor?

Dear all, I am wondering if you have ever experienced bleed-through using the 680and 800 infrared dye secondary antibodies from LiCor. I observed a possible bleed through from a highly expressed...

06 September 2022 1,565 0 View

Does anyone have difficulty in growing bacteria transformed with Lrp6?

I made multiple recombinant Lrp6 with different tags (I used the Gateway system, using a p221Lrp6 and a gateway based expression pCS2 vector). I could get the plasmid with Lrp6-GFP and Lrp6-Flag,...

20 August 2016 9,725 0 View

Does anybody know if there is a Xenopus homologue for the Disrupted in Schizophrenia 1 (DISC1) gene?

I tried to blast zebrafish and mouse protein sequences into Xenopus genome and nucleotide databases but found no correspondence. Is it possible that frogs do not have this gene?

13 October 2015 1,374 4 View

How much dynabeads epoxy m-270 do you use for ImmunoPrecipitation?

After coupling covalently your beads to the antibody of interest, and resuspending the beads at a concentration of 5mg per 165ul (as suggested in the protocol), how much mg/volume of beads do you...

04 January 2015 3,139 4 View

How much antibody do you crosslink to protein A/G beads?

I read protocols suggesting something like 100-150 ug per 250 ul of ptA/G beads (Santa Cruz). I don't have much experience on this but it sounds like a lot. What is your experience? Thank you!

04 January 2015 9,445 5 View

Which golgi marker can I use for co-immunofluorescence?

I need some golgi markers for co-immunofluorescence. In particular, I'd like to see if my gene co-localizes with cis-golgi and TGN (Trans-Golgi network). Would GalNacT2 and TGN38 or 46 be ok for...

14 May 2014 8,141 8 View