How can I calculate azoreductase activity?

I am working on textile dye degradation by bacteria and want to calculate the azoreductase activity (IU/ml or IU/mg protein) from the absorbance value and extinction co-efficient value of...

08 September 2017 2,956 1 View

How to prepare 1000 ppm Lead Nitrate (Pb(NO3)2) solution?

11 December 2016 3,906 6 View

How to fit a standard curve for Cr (vi) reduction assay by DPC method?

09 October 2016 4,861 1 View

Which primer / primer pairs could I use to amplify bacterial azoreductase gene and bacterial chromate reductase gene?

I would like to detect and amplify bacterial azoreductase gene, responsible for azo dye degradation and bacterial chromate reductase gene, responsible for Cr (VI) reduction. I would like to know...

07 August 2016 1,807 1 View

Which are the most effective bioremediation methods of industrial effluent treatment containing dyes and heavy metals?

Thanks in advance

06 July 2016 1,484 3 View

How to determine the gene(s) are responsible for bioconversion of Cr (VI)?

Thanks in advance.

05 June 2016 7,317 1 View

How can I construct a phylogenetic tree?

Need step by step guideline to construct a 16SrRNA phylogenetic tree using MEGA /NCBI-BLAST.

03 April 2016 9,329 6 View

What are the approaches to study genetic diversity in bacteria?

02 March 2016 9,618 2 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View