How can I calculate azoreductase activity?

I am working on textile dye degradation by bacteria and want to calculate the azoreductase activity (IU/ml or IU/mg protein) from the absorbance value and extinction co-efficient value of...

08 September 2017 2,956 1 View

How to prepare 1000 ppm Lead Nitrate (Pb(NO3)2) solution?

11 December 2016 3,906 6 View

How to fit a standard curve for Cr (vi) reduction assay by DPC method?

09 October 2016 4,861 1 View

Which primer / primer pairs could I use to amplify bacterial azoreductase gene and bacterial chromate reductase gene?

I would like to detect and amplify bacterial azoreductase gene, responsible for azo dye degradation and bacterial chromate reductase gene, responsible for Cr (VI) reduction. I would like to know...

07 August 2016 1,807 1 View

Which genes are involved in reactive dye degradation?

Thanks for your feedback

06 July 2016 5,450 4 View

Which are the most effective bioremediation methods of industrial effluent treatment containing dyes and heavy metals?

Thanks in advance

06 July 2016 1,484 3 View

How can I construct a phylogenetic tree?

Need step by step guideline to construct a 16SrRNA phylogenetic tree using MEGA /NCBI-BLAST.

03 April 2016 9,329 6 View

What are the approaches to study genetic diversity in bacteria?

02 March 2016 9,618 2 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How to lessen cell growth?

I am working on the 4T1 murine breast cancer cell line. from the first day I bought this cell line, the cell's growth rate is extremely high. I intend to make these cells resistant to a single...

24 January 2021 2,884 3 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

How can I prepare 300mg/L concentration of lead(II) nitrate ( Mol. Wt reffered is 331.21) incoorporated Luria-Bertani (LB) agar ?

I need to check the Heavy metal (lead) consumption assay using microbes at varying concentration in same media. ( 200mg/L to 2000mg/L).kindly help me to clear it.

19 January 2021 1,946 9 View

Why below-ground ecology is still unexplored (majority of it) in our country ?

Below ground ecology or below ground ecological functions and ecosystems roles are very crucial and highly significant for any ecosystem especially for the forest ecosystems. The interaction among...

02 January 2021 6,912 3 View

What are the role of microbes for green revolution or modern advancement in science ?

We all know the role of microbes its miniature of the miracle how it can be , used in revolutionizing science in various fields.

23 December 2020 6,220 10 View

For a new biofertilizer what efficient methods should I follow to test it's quality(non-presence of pathogens, types &number of symbiotic microbes)?

Also it's effects on plants.

22 December 2020 2,318 3 View