How can I calculate azoreductase activity?

I am working on textile dye degradation by bacteria and want to calculate the azoreductase activity (IU/ml or IU/mg protein) from the absorbance value and extinction co-efficient value of...

08 September 2017 2,956 1 View

How to prepare 1000 ppm Lead Nitrate (Pb(NO3)2) solution?

11 December 2016 3,906 6 View

How to fit a standard curve for Cr (vi) reduction assay by DPC method?

09 October 2016 4,861 1 View

Which primer / primer pairs could I use to amplify bacterial azoreductase gene and bacterial chromate reductase gene?

I would like to detect and amplify bacterial azoreductase gene, responsible for azo dye degradation and bacterial chromate reductase gene, responsible for Cr (VI) reduction. I would like to know...

07 August 2016 1,807 1 View

Which genes are involved in reactive dye degradation?

Thanks for your feedback

06 July 2016 5,450 4 View

How to determine the gene(s) are responsible for bioconversion of Cr (VI)?

Thanks in advance.

05 June 2016 7,317 1 View

How can I construct a phylogenetic tree?

Need step by step guideline to construct a 16SrRNA phylogenetic tree using MEGA /NCBI-BLAST.

03 April 2016 9,329 6 View

What are the approaches to study genetic diversity in bacteria?

02 March 2016 9,618 2 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

What is the role of work experience in motivating high tech entrepreneurship in Aviation industry?

Related work experience plays a vital role in motivating the Entrepreneurship. But when we talk about high tech entrepreneurship in Aviation industry, we talk about very high investment and high...

22 February 2021 376 5 View