What are the approaches to study genetic diversity in bacteria?

More Md. Ekramul Karim's questions See All

How can I calculate azoreductase activity?

I am working on textile dye degradation by bacteria and want to calculate the azoreductase activity (IU/ml or IU/mg protein) from the absorbance value and extinction co-efficient value of...

08 September 2017 3,061 1 View

How to prepare 1000 ppm Lead Nitrate (Pb(NO3)2) solution?

I'd like to know the formula of calculating grams of solute required to make a 1000 ppm solution. Thanks.

11 December 2016 5,093 6 View

How to fit a standard curve for Cr (vi) reduction assay by DPC method?

I'm trying to fit a standard curve for Cr (vi) assay by DPC method. But my curve didn't fit well (R-squared value 0.85). Would you please provide any suggestions for the best fit of the curve.?...

09 October 2016 4,958 1 View

Which primer / primer pairs could I use to amplify bacterial azoreductase gene and bacterial chromate reductase gene?

I would like to detect and amplify bacterial azoreductase gene, responsible for azo dye degradation and bacterial chromate reductase gene, responsible for Cr (VI) reduction. I would like to know...

07 August 2016 1,881 1 View

Which genes are involved in reactive dye degradation?

Thanks for your feedback

06 July 2016 5,541 4 View

Which are the most effective bioremediation methods of industrial effluent treatment containing dyes and heavy metals?

Thanks in advance

06 July 2016 1,570 3 View

How to determine the gene(s) are responsible for bioconversion of Cr (VI)?

Thanks in advance.

05 June 2016 7,387 1 View

How can I construct a phylogenetic tree?

Need step by step guideline to construct a 16SrRNA phylogenetic tree using MEGA /NCBI-BLAST.

03 April 2016 9,474 6 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

How do microbial communities contribute to ecological cycles on Earth and how does an agroecological system support diverse microbial communities?

25 July 2024 5,197 2 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

Bhoj R Singh

Dear you may get some insight at the link below: https://www.researchgate.net/publication/260433705_Microbial_Genetics_Questioned_to_Understand?ev=prf_pub

Book Microbial Genetics Questioned to Understand

Benjamin THOHA Thomas

I guess you mean to say E.coli a not one E.coli, you see different methods can be used depending on your objectives. May if you tell me your objectives, I may be able to guide. But in the absence of that, you can consider the use of RAPD, SSR etc