Which filter size should I use for sterile-filtration of aqueous glycerol solutions (10%)?

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Is there an programm which includes questionnaires, items and picture presenatation?

Dear all, i have to set up a task for facial emotion recognition which combines questionnaires and a picture presentation. Unfortunately i have no programming skills and could not set a program...

10 November 2020 4,516 3 View

Cell structur in poplar - vessels or rays?

Dear All, I am currently trying to understand the different cell types and structures in poplar wood slices, and I have difficulties distinguishing vessel members from rays in the tangential...

12 October 2020 2,276 2 View

Does mysticism have a scientific basis?

Mysticism is often treated as the opposite of science. But is it? Please see Natural Laws and Mysticism in the Cosmic Cycle of Creation

25 July 2020 6,696 80 View

Could our fine-tuned universe have been created from a single fine-tuned quantum particle?

Cosmological explanations for our apparently fine-tuned universe are basically divided between a) a vastly huge multiverse of universes with varying fundamental force and mass constants, including...

21 February 2020 4,618 100 View

In SPSS post hoc results of a 2-way ANOVA, are "Multivariate tests" post hoc tests? If so, can the results be corrected by Bonferroni's correction?

Dear all, I am running a RM ANOVA with 2 independent variables (factor A with 3 levels, factor B with 5 levels). The AxB interaction is significant and I want to know using a post hoc test in...

13 March 2019 7,609 5 View

Does anyone know what is the best way to transfect HEK cells with many constructs (e.g. 15-20)?

I am trying to transfect HEK cells with 15-20 different plasmids and it seems there is some kind of competition for expression. I am currently using Lipofectamine 2000 and transfect each plasmid...

24 February 2019 6,360 3 View

Best RNA extraction method for full-length cDNA?

I'm trying to generate full-length cDNA of my gene of interest (7kb) but have trouble doing so. I was wondering if maybe there are certain RNA extraction methods that are better suitable for this...

29 January 2019 4,774 4 View

How to prepare a 0.75 M sodium citrate solution with pH 7.0?

I want to prepare a TRIzol-like buffer for RNA extraction. In the buffer recipe it's written that I have to prepare a 0.75 M sodium citrate stock solution with pH 7.0. Initially, I only wanted to...

23 October 2018 9,159 6 View

How to make standard curve by using gDNA and plasmid(viral vector) for lenti-virus copy number?

I want to calculate copy number(lentivirus gene in genes of cells) So, i will use two standard curves using WPRE(vector) and Albumin(gDNA) primer. 1. Should plasmid dna be linearized? 2. If so,...

17 June 2018 7,465 3 View

How can I increase protein yield in protein expression?

Hi guys, I'm currently struggling with protein expression... my POI shows very low yield and it seems like the protein expression induction is not very efficient... I already sequenced my...

03 May 2018 9,449 21 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

What are the ranges of four diffterent poles of butterworth filter for ECG signal?

Hi, Can anyone please tell me what are the ranges of four different pole orders such as first order, second order, third order and fourth order respectively of butterworth filter for ECG...

23 February 2021 2,554 3 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

What low-cut filter (high Pass frequency) to be used for response spectrum (Tripartite plots) ?

What low-cut filter (high Pass frequency) to be used for the response spectrum (Tripartite plots)? I am currently working on differentiating between hard/soft site responses in Indo-Gangetic...

21 February 2021 9,273 2 View

Is it possible, for a limited dateset of images, to use Convolution filters + Random Forest instead of UNet?

UNet is 'relatively' recent and was invented mainly to deal with biomedical images, yet is it also possible, for a limited dateset of images, to use Convolution filters + Random Forest instead of...

21 February 2021 4,858 3 View