Which filter size should I use for sterile-filtration of aqueous glycerol solutions (10%)?

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Protocol for neuraminidase treatment of adherent A549 cells?

Dear all, I am looking for a protocol for neuraminidase (NA) treatment of adherent A549 cells (as a control for SNA and MAL-II staining). I am particularly interested in: - Did you fix the...

22 April 2024 9,938 1 View

How do I prevent dark blue spots/smear and the running front of my BN-PAGE?

Hi everyone, we are trying to establish BN-gels in our lab and we are currently facing some problems. We would like to investigate protein-protein interaction of membrane bound proteins from cell...

18 April 2024 3,219 0 View

Could the DNA/RNA genetic code have existed in the early stages of the Big Bang?

No one knows the origin of the genetic code for DNA/RNA observed in all living organisms on Earth. Current scientific thinking is that the genetic code evolved naturally during the abiogenic...

14 September 2023 2,288 4 View

Are cells of the human ovarian cancer cell line HEY positve for the surface marker EpCAM?

Hello everyone, I'm currently establishing a new project using the human ovarian cancer cell line HEY. Unfortunately it turned out that they are negative for both of the two surface markers...

11 September 2023 6,341 2 View

Peppa Pig's episodes transcripts in different languages?

Hi! Does anyone knows if there's a website where I can find Peppa Pig's episodes transcripts in different languages? Or even better, do you know if a parallel corpus including these already...

07 December 2022 1,183 0 View

How to interpret a log transformed dependent variable and a non-transformed independet variable when beta is high?

Dear all! i am not sure how to interpret a log transformed dependent variable Y and a non-transformed independet variable X when beta is high. In my example the regression coefficient (beta) of...

09 November 2022 1,128 3 View

Sample runs out after starting PAGE?

After starting the electrophoresis my samples often 'fall' trough the pocket of the gel and accumulate on the stacking/resolving gel border. Every ingredient of the gel had been prepared fresh,...

07 November 2022 6,489 1 View

Can mycoplasma-infection or treatment with plasmocine affect stem cell differenciation and ion channel functions?

Hello, we had mycoplasma in our stem cell and cardioymyocyte cell culture and after treatment the cells growed very slowly/some were dying (picture) and most differenciations were no longer...

01 March 2022 6,255 2 View

How to stain undifferentiated THP-1s (or other suspension cells) for immunofluorescence microscopy?

Dear everyone, I want to label THP-1 cells for IF microscopy but cannot find any detailed protocol on how to label suspension cells without cytospin - is there at all a way of labelling...

13 December 2021 5,302 3 View

Best way to harvest cells from gelatin-coated plates?

Hello! I'm trying a new protocol, and will be seeding isolated mouse endothelial cells on a 0.1% gelatin-coated 12-well plate. I was wondering if there were any established methods to harvest the...

11 November 2021 9,974 1 View

Is the black sediment consider as sand? If not, how can I filter it out?

Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...

30 July 2024 557 2 View

What should be the sample container for the hydrothermal reaction in a microwave reactor at 180 °C for 10 min at the heating rate of 5 °C per minute?

Suggest one of them 1. Teflon-lined stainless steel autoclave: 2. Alumina (Al2O3) ceramic container

30 July 2024 7,326 1 View

Can we isolate microplastic from sludge sample without using vacuum filter..?

isolation of microplastic from sludge sample using centrifugation ..

23 July 2024 6,418 0 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

I want to buy Hydrothermal Synthesis Autoclave from any European company. Can anyone suggest any company inside Europe?

Thanks

22 July 2024 1,143 3 View

In kalman filter, it is logical to have the observation variance equale 0 ?

I am working on implementing a Kalman filter integrated with ARMA parameters, as described in the article "Predicting Time Series Using an Automatic New Algorithm of the Kalman Filter"...

12 July 2024 8,116 2 View

How to wash capsule filters (Membrane: polyethersulfone; pore size0.2u/0.45u) after filtration. can I wash by NaOH? if yes, what will be the molarity?

11 July 2024 8,969 1 View

Is it necessary to autoclave every vessel used in DNA isolation?

DNA isolation requires many freshly made buffers. So is it necessary to autoclave each vessel used, before preparing buffers in them?

10 July 2024 7,526 3 View

How to filter structural variants from WGS/WES data?

Hello everyone! I would appreciate some advice regarding the filtering of structural variants. The variant caller used for discovery-“DELLY or DELLY2”using whole-genome sequencing and Whole...

09 July 2024 498 1 View

How to filter/get the silver nanoparticles &nanoplates ? We synthesis this silver nanoparticles materials from the NaBh4, trisodium citrate,H2O2?

After finish the synthesis of nanoparticles.and nanoplates of silver how to take out those as solid/powder form from the solution. Please give a solution of u already done that and succeeded by that

27 June 2024 692 1 View