Review and cite FILTERING protocol, troubleshooting and other methodology information | Contact experts in FILTERING to get answers
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Questions related to Filtering
Has anyone else run Westerns for LC3A/B with HSV-1 infections in human and mouse glial cells and seen little to no LC3A/B expression with viral MOI above 1? I used MOI 1, 2, and 5 a few times and...
25 January 2024 8,182 0 View
What is the microplastics cycle?
24 January 2024 8,917 1 View
I need to know to which taxonomic levels (from phylum to species) the following approaches allow prokaryote identification: a) morphology-based approaches b) chemotaxonomy c) GC content d)...
24 January 2024 4,389 6 View
I had set up overnight ligation reaction at 16 degree celsius in a PCR machine but the lid temperature was high around 95 degrees. Will it affect the efficeincy of ligation if done in PCR in this way?
23 January 2024 2,410 2 View
Imagine you have a field with excessive salt buildup. Assume the volumetric water content is 0.35 cm3 cm-3 and the root zone is 120 cm deep. If the soil in the field is a clay loam soil with Ks=...
21 January 2024 6,323 4 View
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20 January 2024 8,847 2 View
Waste management and green supply chain.
12 January 2024 8,539 4 View
Usually, my ecoli colony has a distinct shape and doesn't form a hairy look like this. I use LBA with AMP 100 µL/mL. The plasmid inside my ecoli has the AmpR region so they should survive under...
11 January 2024 9,407 2 View
Hello everybody, I have a question related to western blot. I'm studying the activation/phosphorylation of p65-NFKB in monocytes over time using three different stimuli. The transfer went well...
11 January 2024 7,766 6 View
I want to fit the Havriliak-Negami function for real and imaginary dielectric values in MATLAB or Origin software. Can anyone suggest the build-up equation? and how do I put initial guesses?
11 January 2024 5,339 1 View
Explore cutting-edge digital forensics research trends, focusing on tech impact, challenges, and innovative responses to evolving cyber threats.
10 January 2024 4,432 2 View
An incorrect research has been added to my profile under my name and I wish to delete it.
11 December 2023 9,913 0 View
Ongoing community Piggeries of Leguminous Hog feeds enzyme based model.
03 December 2023 5,453 0 View
Is magnetic field always perpendicular to electric field and what are the electric and magnetic field vectors in electromagnetic waves?
01 December 2023 3,307 2 View
How important do you consider it is, for a math student, to take part in competitions? Why?
16 November 2023 5,336 1 View
I have two groups of mice representing WT and knockouts. Each mice group (WT and KO) will be allocated to 4 experimental categories. I need to allocate these available mice into these experimental...
14 November 2023 8,530 1 View
Adsorption process for a good isotherms, kinetics and rate of reaction
12 November 2023 5,442 0 View
Hi, When making liposomes, the lipids in organic solvent are first put in a round-bottom flask and are 'rotated' while getting dried under nitrogen gas to obtain "a uniform thin film" of lipids...
11 November 2023 9,845 1 View
I would like to simulate a passive-quenching active-reset circuit for single-photon avalanche diodes (SPAD) for a project. Is there any SPAD model available online that I can import for the...
10 November 2023 5,874 1 View
in statistical data,why we use this? how to calculate a confidence interval in data like 25.5_27.3
06 November 2023 9,232 2 View
I am performing siRNA transfection to inhibit a certain matrix RNA. Does it make sense in this case to determine the reduction of this RNA by reverse transcription on PCR?
31 October 2023 1,431 4 View
should we technically fix the exosomes using solution like PFA for TEM analysis? (is fixation necessary just for checking the morphology of exosomes by TEM?))
31 October 2023 2,827 1 View
I want to use Pioloform-cupper (agar scientific) TEM grids for exosome analysis. Should we hydrophilize these grids prior to sample preparation?
31 October 2023 7,300 0 View
I need to carry out corrosion tests on steel in a three electrode cell using a sulfuric acid solution. Which reference electrode do you recommend I use?
25 October 2023 9,505 0 View