Review and cite FILTERING protocol, troubleshooting and other methodology information | Contact experts in FILTERING to get answers
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Questions related to Filtering
Hi, everybody. I'm coming today with a technical question. I'm having problems culturing HEK293 cells. Under the same culturing conditions, my HEK293T cells survive after splitting while the...
22 August 2023 6,827 8 View
I have attempted various transfection reagents including Lipo3000/Lipo2000/Hieff Trans™ Liposomal Transfection Reagent, both GAPDH positive control and custom siRNA targeting gene of interest at...
21 August 2023 6,057 2 View
I'm in the process of creating classification models using a substantial dataset (approximately an MxM matrix). To enhance the performance of these models, I'm planning to conduct some feature...
18 August 2023 4,704 5 View
It seems that gelatin is separating from its solution with PEG before forming gel. Is there any way to create a stable gelatin-PEG solution/gel? I'm using PEG-400.
16 August 2023 3,178 3 View
I wanted to calculate the Nusselt Number in tetrahedral and hexahedral mesh with the same grid size. As in tetrahedral mesh the grid size is more. When doing for the hexahedral mesh, the element...
14 August 2023 4,210 0 View
Hello. I am working on heterologoulsy expressing and purifying a protein using the Bac-to-Bac method. The protein expresses as verified by western blotting. I typically do a 4.2 L expression...
14 August 2023 7,694 9 View
I have received the sequence of plasmid, I aligned it with the gene sequence on APE plasmid editor and it is matched. But when I performed vector screening on ncbi, the sequence is strongly...
14 August 2023 1,887 2 View
What are the applications of an embedded system and what is classification and requirements of embedded system?
13 August 2023 3,967 2 View
Hi All and thanks in advance. For long shelf life cleaning solutions, what concentration of protease and what stabilising agent should be added to hypochlorite solution content to preserve the...
08 August 2023 304 3 View
Nowadays I see many DVBT antennas on the market that announces to have LTE or 5G filters. Is there any techniques to do filter in the yagi design (parasitic element to filter specific frequencies)...
04 August 2023 4,031 3 View
I am working with a protein located in the mitochondrial inner membrane, and I would like to know in which conditions should I perform the denaturation step... maybe the "standard" denaturation at...
03 August 2023 4,110 3 View
What are the environmental conditions needed by living things to survive and relationship between organisms and their environment?
30 July 2023 3,301 3 View
When we go for analysis with likert scale data we have to measure chronbach alpha to know the internal consistency about the response.But what is the actual difference between validity,...
15 July 2023 6,091 3 View
What is the name of the programs used to make images/synthesis figures/experiments/methods?
30 June 2023 5,658 0 View
If I have chosen seven different types of sites (Commercial, residential, industrial, transport, and background) within a city for conducting source apportionment using PMF, what is the minimum...
27 June 2023 3,138 0 View
What is the best way to synthesize cDNA; using poly A kit or stem loop PCR? Why is that
14 June 2023 4,548 4 View
What are the key technical challenges associated with integrating a solar field into an already operational combined cycle gas turbine power plant, and how can these be addressed in a...
11 June 2023 9,142 1 View
I am applying periodic boundary conditions on the matrix phase (without inserting any fibers into it) and calculating the effective Young's modulus in X direction. In the property module, Youngs...
10 June 2023 8,434 3 View
I try to use (http://www.harzing.com/pop.htm) to export the results of the Google Scholar search to Excel, but it's not working.
08 June 2023 9,693 0 View
I am planning to cultivate algae-bacteria biofilms under greenhouse conditions using biofilm carriers. I have routinely come across Industrial Soft Carriers as frequently used substrates for...
07 June 2023 2,815 1 View
I'm a beginner and would like to ask you some questions about identifying nematodes in soil: How do I preserve soil samples? 4°C or -20°C? What are the steps to identify nematodes with a DNA...
07 June 2023 8,951 4 View
I read somewhere that a delayed version of the noisy signal can be used as the reference signal but then how can we get a clean signal from giving a noisy signal as reference?
26 May 2023 5,671 0 View
hello, I'm using about 50 gene trees to infer the species tree for each gene I have performed IQTREE with each gene (8 species, 10 sample for each species) and got the gene tree for each gene How...
22 May 2023 1,164 0 View
I want to make a research on Effects of Partnership on Reading Remediation to raise level of Non Readers
18 May 2023 4,543 2 View