Review and cite FILTERING protocol, troubleshooting and other methodology information | Contact experts in FILTERING to get answers
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Questions related to Filtering
Dear colleagues, I am working with FLAC (dynamic analysis using PM4Sand) and I would like to ensure that the static stage of my model is already stable before applying the dynamic loading. In my...
last month 1,226 4 View
I've been trying to purify two full-length proteins (N-His and C-His tagged) for several months, but I keep encountering degradation during the purification process. On SDS-PAGE, the proteins are...
24 July 2025 6,736 3 View
Hello everyone, I’ve just uploaded my latest work: “Lung Nodule Segmentation: Exploring Data Efficiency and Advanced Architectures” Lung Nodule Segmentation: Exploring Data Efficiency and...
26 May 2025 7,044 3 View
I have mitochondrial protein coding genes aligned for individual genes like 12S-aligned where I have just 12S aligned gene for all the species.Now I'm confused how to concatenate the genes for...
14 May 2025 9,656 0 View
I have performed several enzymatic: lipolytic, amylolytic, and proteolytic activity assay. After looking into it, it turns out there are three formulas for calculating the hydrolytic index. For...
24 April 2025 9,472 1 View
In feature fusion, should we extract features from the same (seen) data used to train the base models, or from unseen data? Since these features are later used to train a final classifier, I’m...
05 April 2025 4,235 0 View
I'm trying to purify the TCF3 protein (~70-75kda kDa, pI 4.5) from E. coli BL21 cells after inducing expression with 1 mM IPTG. I am using Ni-NTA affinity chromatography to purify the His-tagged...
22 March 2025 1,787 3 View
How to get predicted head observation values in Modelmuse - MODFLOW groundwater modelling? We have already run the model by selecting the " prediction" option available. However we are not getting...
18 March 2025 179 1 View
Hello all, I expressed my fusion protein bound to MBP using a pMAL-c2e vector and purified it using an amylose column. Upon running a cleavage reaction of 1 mg of my fusion protein in 1 mL of 20...
17 March 2025 6,918 0 View
I have been dosing dissolved silica content in my samples by ICP. However, I often obtain more than 100% of silica extraction after treating the data ... I wonder what would be more suitable for...
02 March 2025 1,335 1 View
I installed AutoDock Tools in my Windows 11 systems, a few day back and it was working well, then after some time i noticed that the logo symbol from the desktop was gone, and now when im trying...
19 February 2025 5,893 1 View
I am researching quantum kernel methods for classification tasks, focusing on mapping classical data into high-dimensional Hilbert spaces using parameterized quantum circuits. My goal is to design...
07 February 2025 5,888 1 View
I am an editor at a scientific journal Progressive Economy. How can I upload articles by our authors from my account? I am not the author of the articles, but I have the right to post these...
12 January 2025 4,746 1 View
the concept is related to the topic, diffusion of innovations.
16 December 2024 4,467 1 View
What specific benefits has artificial intelligence brought to business management practices, and how can organizations effectively leverage these advantages to enhance their operations and...
30 October 2024 6,300 4 View
When trying to know the effect on T2Diabetes on human using albino rat
22 October 2024 3,862 1 View
Dear All: I want to quantify the fluorescence intensity of images using fluorescence microscope. I would really appreciate it if someone could tell me how to use image j correctly.
09 September 2024 5,770 1 View
I've cultured cells "cell lines" (stored for a long period in liquid nitrogen). I have a problem with cell attachment, as most cells are still in suspension and some form spheroids. The cells tend...
15 August 2024 2,758 3 View
Collagenase / Accutase or other enzyme digestion is often used method for tissue digestion. However, in contrast to mechanical digestion it could lead to significant reduction in the expression...
23 July 2024 9,667 1 View
Hi, Would anyone have already seen such black swimming rods in cell culture (see picture). Here are their key features: - The medium never turns turbid; - At first, cultured cells look OK, but...
24 June 2024 9,444 2 View
Hello everyone. I cultured isolated PBMCs in Erythroid Expansion Medium for two weeks and then performed electroporation in my expanded PBMCs in order to reprogram them. The first 24h after the...
23 June 2024 432 4 View
I have searched a lot of information from the published article but couldn't find valuable. As I have prepared the Lentiviral vector and stored it in -80 degree!
22 June 2024 3,551 1 View
Dear all, For higher biomass for saccharomyces cerevisiae which media is suitable in shake flask? 1. YPD media 2. Basal salt media and what is the optimum C/N ratio ( 5:1 /10:1). Thanks &...
19 June 2024 2,244 0 View
till now, there are many superconductors. Is there any relationship between critical temperature of superconductor and the atomic weight & radius? Now the isotope effect is one relationship...
13 June 2024 2,154 3 View