Review and cite FILTERING protocol, troubleshooting and other methodology information | Contact experts in FILTERING to get answers
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Questions related to Filtering
Hi, Would anyone have already seen such black swimming rods in cell culture (see picture). Here are their key features: - The medium never turns turbid; - At first, cultured cells look OK, but...
24 June 2024 9,313 2 View
Hello everyone. I cultured isolated PBMCs in Erythroid Expansion Medium for two weeks and then performed electroporation in my expanded PBMCs in order to reprogram them. The first 24h after the...
23 June 2024 263 4 View
I have searched a lot of information from the published article but couldn't find valuable. As I have prepared the Lentiviral vector and stored it in -80 degree!
22 June 2024 3,400 1 View
Dear all, For higher biomass for saccharomyces cerevisiae which media is suitable in shake flask? 1. YPD media 2. Basal salt media and what is the optimum C/N ratio ( 5:1 /10:1). Thanks &...
19 June 2024 2,022 0 View
till now, there are many superconductors. Is there any relationship between critical temperature of superconductor and the atomic weight & radius? Now the isotope effect is one relationship...
13 June 2024 1,823 3 View
As you can see below, I cloned piggybac transposon vector which have two independent promoters (CMV-A, B, C gene / EF1a core-PuroR), but it didin't work well. After Electroporation with...
06 May 2024 9,298 0 View
Can biochar increase the bioavailability of phosphorus and will it be safe to apply biochar into soil for large scale especially agricultural soil?
21 March 2024 9,330 0 View
Hi everyone, the x=axis is samples sample1= wt sample2= yk∆ sample3=swe∆ So, what is the right code for this symbol ∆? Thank you
17 February 2024 6,031 0 View
I would like to find the papwe published by IEEE about unite element transmissionline and lattice filte in 1980-1990.
05 February 2024 278 0 View
Dear all, During my electrochemical reaction, I used tetrabutylammonium chloride (TBACl) as an electrolyte in N,N' dimethyl formamide (DMF) solvent. During product analysis by NMR, I faced...
01 February 2024 8,893 0 View
Has anyone else run Westerns for LC3A/B with HSV-1 infections in human and mouse glial cells and seen little to no LC3A/B expression with viral MOI above 1? I used MOI 1, 2, and 5 a few times and...
25 January 2024 8,227 0 View
What is the microplastics cycle?
24 January 2024 8,978 1 View
I need to know to which taxonomic levels (from phylum to species) the following approaches allow prokaryote identification: a) morphology-based approaches b) chemotaxonomy c) GC content d)...
24 January 2024 4,438 6 View
Good day everyone, I would like some help in getting online references to answer the question I have posed. I am dithering and running from pillar to post to find answers. Many...
24 January 2024 6,215 2 View
I had set up overnight ligation reaction at 16 degree celsius in a PCR machine but the lid temperature was high around 95 degrees. Will it affect the efficeincy of ligation if done in PCR in this way?
23 January 2024 2,478 2 View
Imagine you have a field with excessive salt buildup. Assume the volumetric water content is 0.35 cm3 cm-3 and the root zone is 120 cm deep. If the soil in the field is a clay loam soil with Ks=...
21 January 2024 6,367 4 View
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20 January 2024 8,905 2 View
(I mean the generalized Minkowski's spacetime $R^{n+1}$)
14 January 2024 817 0 View
Waste management and green supply chain.
12 January 2024 8,598 4 View
Usually, my ecoli colony has a distinct shape and doesn't form a hairy look like this. I use LBA with AMP 100 µL/mL. The plasmid inside my ecoli has the AmpR region so they should survive under...
11 January 2024 9,449 2 View
Hello everybody, I have a question related to western blot. I'm studying the activation/phosphorylation of p65-NFKB in monocytes over time using three different stimuli. The transfer went well...
11 January 2024 7,824 6 View
I want to fit the Havriliak-Negami function for real and imaginary dielectric values in MATLAB or Origin software. Can anyone suggest the build-up equation? and how do I put initial guesses?
11 January 2024 5,382 1 View
Explore cutting-edge digital forensics research trends, focusing on tech impact, challenges, and innovative responses to evolving cyber threats.
10 January 2024 4,472 2 View
An incorrect research has been added to my profile under my name and I wish to delete it.
11 December 2023 9,953 0 View