Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

Can someone explain blocking vs. nonblocking electrodes in terms of electrochemical impedance spectroscopy?

I have fabricated thin films and want to measure the impedance parallel to the material. I suspect the films are conducting protons and electrons based on some Hall effect measurements I have...

27 January 2021 1,712 5 View

Isoabsorbance and 3D Plots are missing under the "Spectra" toolbar of Agilent Chemstation 1200 series software. How can I retrieve it please?

The Problem started after replacing the HPLC Pump from Quaternary to Isocratic pump due to pump failure. The configuration was set as the previous set up as Modular 3D LC System and the license...

11 October 2020 8,018 3 View

I am using a rat TNFa ELISA to measure TNFa in culturing media. How soon following my alcohol treatment should I collect and measure TNFa?

I am using an organotypic hippocampal slice culture where we are exposing our slices to alcohol with subsequent alcohol withdrawal. I want to make sure I am capturing the right time to assess...

16 September 2020 7,020 2 View

When is simulation necessary in copula modeling?

When modeling dependencies using copulas, when is simulation necessary? Kindly need expert advice on this.

08 June 2020 7,166 3 View

Has anyone using organotypic hippocampal slice culture models observed holes in their tissue specimens between 6-12 days in vitro?

Our lab has noticed that after several tissue preparations for organotypic hippocampal slice cultures, some of our tissue is developing what appears to be large holes between the CA1 and dentate...

03 March 2020 3,981 3 View

How do I graph RT-PCR amplification values?

I have conducted an experiment to investigate the up-regulation and down-regulation of immune related genes. I have calculated the PCR efficiency and used the pfaffl method to get amplification...

22 October 2019 1,231 1 View

Is Learning Analytics under Data Mining?

In what ways EDM and LA differ and does LA under Data Mining and does it use the same techniques from Data Mining?

30 April 2019 8,370 1 View

No title

13 December 2017 8,042 3 View

How do the cross terms affect accuracy in a high order CFD code?

If you drop the grid spacing down to where it's tiny, you'll get the time marching errors, and if you drop the time step, you'll usually get the error of differencing terms. How do you quantify...

18 October 2016 9,151 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View