. Which DNA is restricted to making a genomic library?

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How has recombinant DNA technology contributed to the development of genetically modified organisms (GMOs)?

I want to know some ways in which it has contributed to their development.

19 June 2023 9,763 3 View

How can you ensure the specificity and reliability of Western blot results?

what are the key steps included in this to ensure specificity and reliability

14 June 2023 5,279 4 View

Is there a fluorescent protein with an emission max greater than 720 nm? Best fluorescent protein with an emission max >700nm for making BRET sensor?

Does anyone know of a fluorescent protein with an emission max greater than 720 nm? Which is the best fluorescent protein with an emission max greater than 700 nm for making a BRET sensor? I have...

17 May 2023 1,144 9 View

How to calculate effective thermal conductivity of bent heat pipes?

Hi, I am trying to perform CFD/FE thermal analysis of grooved heat pipes by specifying the effective thermal conductivity of the heat pipe When we are specifying effective thermal conductivity,...

11 May 2022 6,432 3 View

What are some research topics on full stack development?

Latest full stack development topics to research

17 September 2021 6,437 1 View

After performming the below protocol the percentage of recombinance I got is 60%. How can I extend the percentage of recombinance to 90?

How can I increase my recombinance

20 August 2015 9,060 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View