I am working with B. thuringiensis.
I have isolated about 50 B. thuringensis cultures from different regions of Marathwada, India. Now I have to check the presence of a specific cry gene which has been least studied by other researchers.
Hi, did you check the literature? Normally there is given such information! Cheers, Nadine
ya I have read out. mostally cry I and II gene study is done, as its percentage of occurence is high.
Hello Kunal,
The following papers talk about new cry genes and for sure, all of them won't be studied in detail. You can go through them and do a literature search with them.
- Detection of New cry Genes of Bacillus thuringiensis by Use of a Novel PCR Primer System[down-pointing small open triangle]
Pedro A. Noguera and Jorge E. Ibarra*
Appl Environ Microbiol. Sep 2010; 76(18): 6150–6155.
- Mining new crystal protein genes from Bacillus thuringiensis on the basis of mixed plasmid-enriched genome sequencing and a computational pipeline.
Appl Environ Microbiol. 2012 Jul;78(14):4795-801. doi: 10.1128/AEM.00340-12. Epub 2012 Apr 27.
Best
Simran
I serve on the Bacillus thuringiensis Toxin Nomenclature committee. For an up-to-date list of all named and sequenced Cry proteins, please check the nomenclature page maintained by Neil Crickmore at the University of Sussex (see link below). Most cry genes/Cry proteins are not very well characterized. The most thoroughly studied are those with current commercial applications, especially the Cry1A proteins, Cry2Aa, certain Cry9 proteins, and the mosquitocidal proteins from B. thuringiensis serovar. israelensis (Cry4, Cry10). Feel free to contact me for information about any specific protein.
http://www.btnomenclature.info/
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