Which control should be used for my calculation: a positive which has standard compound or a negative control which has reagents only

More Disha Nayak's questions See All

Hello my rat adipocytes are not getting attached (passage 3 & 4) after I'm reviving them. What could be reason? Any idea to get them attached?

It's been more than 20 days they are not getting attached to surface of flask.

04 March 2024 8,594 0 View

Hello, my project involve rat adipcytes, isolating from adipose tissue, It's getting turbid contamination. Can someone help me with procedure?

At first try, I got the cell but after that I'm not getting the cell. Can someone help me where I must be going wrong during the procedure.

04 March 2024 3,408 1 View

Why is my western blot not transfering straight?

I have human brain tissue samples that I lyse with a nuclear extraction buffer and then the pellet I solubilized in 8M urea. I run samples in Tris-Glycine buffer in premade 4-12% gels. Ran the gel...

04 March 2024 9,722 0 View

Which media is used for culturing batrachospermales ?

I have a fresh water red algae. Which media will be suitable to grow it ?

26 February 2024 8,391 1 View

Can anobody provide me the absorption files of different hole and electron transport layers for using in SCAPS-1D simulation?

I'm working with SCAPS-1D, where I've run the simulation without absorption files of the layer materials. But for better accuracy, I need the absorption files for all the possible HTLs and ETLs...

26 February 2024 7,522 3 View

Can any one tell me Environment education related journal?

I am interested in learning about environmental science, climate change, and sustainable development concepts in children's and teachers minds and would like to do some research and review on the...

21 February 2024 2,113 3 View

Can anybidy tell me the UGC care general in Education field?

Need some quick publishing reputed name of journal for education, Science education field

07 February 2024 4,799 1 View

Why the composites exhibited a hysteresis M-H loops since the NiO is antimagnetic?

NiOCNF added with PVDF matrix , why its showing the Ferrimagnetic behaviour

15 January 2024 8,433 0 View

Missing operator Errors in VMD pathways plugin?

Hi, I'm using the VMD pathways plugin for the first time. I'm analyzing a trajectory, these are few issues I'm facing while doing it. I'll be grateful for any insight. 1. When I'm giving the...

11 December 2023 6,636 0 View

In the absorber layer of the solar cells, what is the intensity of the photoluminescence spectra?

If we use undoped materials and record their PL intensity, the intensity of the sample rises or decreases after doping. We need a low rate of recombination with a higher charge carrier...

04 December 2023 2,368 1 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Are there any good simple systems or platforms to recommend?

In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?

05 August 2024 10,034 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Are current regulations effective in preventing cancer caused by toxins?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 4,474 2 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

How I can add anew research in my account ?

Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?

21 July 2024 7,545 0 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Ke-Wei Zhao

Disha: You have to be mindful that all your numbers are net readings (e.g., OD of control minus OD of reagent only, OD of sample minus OD of reagent only, etc). Then your equation should be fine.

Rajesh K P

At is of control you have to put the respective od value what you get for the reaction mixture without any test sample. I mean Zero concentration tube