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Questions related from Rajesh K P
Dear all With respect to above mentioned subject, we use tricine SDS electrophoresis to resolve small peptides. Earlier separation was very good and getting sharp bands. Now we are facing problem...
07 August 2017 3,091 8 View
Hello friends. I am optimizing the method for estimating gene copy index by using Beta actin as house keeping gene. In each trail using same genomic DNA, I am getting different CT value. Can any...
07 December 2015 795 6 View
I isolated some compounds from plant bark extract. I took NMR, IR, Mass, everything. I am able to analyse the spectras also. But I don't know on what basis we need to arrange the molecules to get...
06 November 2013 6,789 3 View
I did column chromatography to separate the compounds present in plant extract using sephadex LH 20 as stationary phase and 100 % as the solvent system. Now I need to find out whether the elute...
18 August 2013 5,359 2 View
Can anyone tell me what the formula to calculate the total protein carbonyl content in tissue homogenate is? Is it the same as MDA calculation using absorption coefficient value (22,000/M/Min)?
07 August 2013 5,636 16 View
I prepared liver section and also took photographs. Now I don't know how to analyse the histology. What are the parameters we need to study including necrosis and hemorrhage? How to find out...
01 August 2013 4,226 6 View
I am working on natural products and I am facing too many problems while separating compounds from crude plant extract. I tried all the ways, but it was no use. Now the ultimate step is to...
22 March 2013 3,557 17 View
Before going to extraction with polar solvents, we need to defat the plant materials with lw polar solvents like petroleum ether. My question is, what are the possible secondary metabolites to...
21 March 2013 4,132 6 View
