I am looking for ordinary 96 well tissue culture plates, but with glass bottom. Please help.
I have 2 cell groups; (WT)Wild-Type (Subject)Wild-Type+Transfected Gene I expose these cells to a particular drug at different doses. I then look at calcium influx. The drug is known (has...
04 May 2019 2,357 4 View
I have 3 cells lines; Wild type control Wild type transfected with GFP vector Wild type transfected with GOI-GFPtag I'd like to do some general characterization on the cells, including cell...
05 June 2018 9,918 0 View
How vital is it to optimize the RNA input for reverse transcription for subsequent qPCR reactions? The qPCR reaction mix protocol calls for 25ng of template. I've been using 100ng total RNA for...
02 March 2018 587 5 View
I'm considering performing a PCR on my gene sets (both GOIs and house keeping genes), run the PCR products on a gel, extract them from the gel, and then measure their concentrations (using a...
02 March 2018 9,662 5 View
I have plasmid containing my gene of interest. It has been linearized, gel purified, and is at known concentration. I will use this standard to generate a standard curve for my qPCR runs, and...
02 March 2018 9,149 4 View
I'm very familiar with neuroblastoma cell lines, both general culturing, and differentiating. However, I've been considering transfecting my gene of interest into a non-cancer cell line. What are...
02 March 2018 5,908 2 View
I have RNA samples that I will be using for cDNA synthesis, and analysis of expression levels. The RNA has been DNAse treated, both in column during extraction, and post column extraction...
02 March 2018 773 1 View
I have acquired a lot of images of my neuron's axons degenerating over time by various different insults. A lot of my data is qualitative, and I could perhaps go through all the images and count...
01 February 2018 2,276 2 View
I grow my neurons on glass surface. Their axons so delicate that I cannot remove the media entirely. When I refeed them, I remove half the media and replace it with fresh media containing double...
01 February 2018 1,768 3 View
I've added 6-OHDA to my neuronal cultures to test whether they are dopaminergic, and they do die. However, I was told that I need to also inhibit noradrenaline reuptake trasnporters as 6-OHDA is...
31 December 2017 9,903 2 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...
01 March 2021 395 3 View
Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !
01 March 2021 2,501 3 View
We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...
28 February 2021 1,149 1 View
Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...
28 February 2021 7,292 3 View
I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...
27 February 2021 6,801 3 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View