I am looking for ordinary 96 well tissue culture plates, but with glass bottom. Please help.
I have 2 cell groups; (WT)Wild-Type (Subject)Wild-Type+Transfected Gene I expose these cells to a particular drug at different doses. I then look at calcium influx. The drug is known (has...
04 May 2019 2,462 4 View
I have 3 cells lines; Wild type control Wild type transfected with GFP vector Wild type transfected with GOI-GFPtag I'd like to do some general characterization on the cells, including cell...
05 June 2018 10,024 0 View
How vital is it to optimize the RNA input for reverse transcription for subsequent qPCR reactions? The qPCR reaction mix protocol calls for 25ng of template. I've been using 100ng total RNA for...
02 March 2018 686 5 View
I have plasmid containing my gene of interest. It has been linearized, gel purified, and is at known concentration. I will use this standard to generate a standard curve for my qPCR runs, and...
02 March 2018 9,255 4 View
I'm considering performing a PCR on my gene sets (both GOIs and house keeping genes), run the PCR products on a gel, extract them from the gel, and then measure their concentrations (using a...
02 March 2018 9,765 5 View
I'm very familiar with neuroblastoma cell lines, both general culturing, and differentiating. However, I've been considering transfecting my gene of interest into a non-cancer cell line. What are...
02 March 2018 6,003 2 View
I have RNA samples that I will be using for cDNA synthesis, and analysis of expression levels. The RNA has been DNAse treated, both in column during extraction, and post column extraction...
02 March 2018 860 1 View
I have acquired a lot of images of my neuron's axons degenerating over time by various different insults. A lot of my data is qualitative, and I could perhaps go through all the images and count...
01 February 2018 2,578 2 View
I grow my neurons on glass surface. Their axons so delicate that I cannot remove the media entirely. When I refeed them, I remove half the media and replace it with fresh media containing double...
01 February 2018 1,898 3 View
I've added 6-OHDA to my neuronal cultures to test whether they are dopaminergic, and they do die. However, I was told that I need to also inhibit noradrenaline reuptake trasnporters as 6-OHDA is...
31 December 2017 9,988 2 View
I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?
08 August 2024 8,915 0 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)
06 August 2024 6,610 4 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...
16 July 2024 5,047 0 View