Which commercially available Pyrophosphate(PPi) Detection Kit works best for cellular level PPi detection?

08 August 2014 1 8K Report

I have to detect PPi levels in multiple cell (mammalian) lines.

Pyrophosphate (PPi) Assay Kit (ab112155). Direct fluorometric quantification of pyrophosphate in cells.

Pyrophosphate Assay Kit (Fluorometric) provides the most robust spectrophotometric method for the measurement of pyrophosphate. It uses a proprietary fluorogenic pyrophosphate sensor that has its fluorescence intensity proportionally dependent upon the concentration of pyrophosphate. Abcam's assay is much easier and more robust than enzyme-coupling pyrophosphate methods, which require at least two enzymes for their pyrophosphate detections. Due to its direct measurement of pyrophosphate, this kit is ideal for screening inhibition or activities of enzymes that consume or generate pyrophosphate. The assay is an optimized mix-and-read assay and can be performed in a convenient 96-well or 384-well microtiter-plate format. The kit provides all the essential components for assaying pyrophosphate.

http://www.abcam.com

Badges
Science topic

More Gunjan Pandey's questions See All

What is the best method to dissolve curcumin in DMSO?

04 May 2016 4,898 1 View

Can anyone please suggest if other than PBS and HEPES there is any other buffer that can be used for washing HeLa cells?

I have been conducting an experiment on HeLa cells; I do not need phosphate contamination or at least minimize phosphate interaction with my culture as much as I can. Thanks

05 June 2014 7,035 5 View

Can anyone explain in detail the difference between Ferric Citrate and Ferric Ammonium Citrate?

I was able to find that Ferric citrate provides iron in less toxic form than other free iron salts but was not able to find any detail on either how such mechanism is possible.

04 May 2012 6,350 0 View

Can anyone explain the difference between Bile Esculin Agar and Esculin Iron Agar used in Esculin Hydrolysis Test?

The composition of both the Chemicals is almost same except for the presence of Ferric Citrate in the former and Ferric ammonium citrate in the latter.

04 May 2012 7,644 0 View

How does increased NaCl concentration in culture broth effect the growth Vibrio Cholera?

Gram negative bacterial culture.

03 April 2012 7,892 22 View

Does anyone knows the complete protocol for arginine dihydrolase test done for Vibrio cholera identification?

I have found several links but none of them provide the whole experiment. There is one done by Thornley M.J. (1960) but I am not able to access it. Kindly help me out. :(

01 February 2012 6,386 2 View

Kindly help me with some protocols on spectrophotometric analysis of amino acids..As my institute doesn't provide me with HPLC and other equipments..

I need to conduct an experiment on identification of certain amino acids.. I will be highly gratified to have some valuable suggestions.. as I don't have an option due to lack of resources.. :(

31 December 2011 9,156 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View