I need to conduct an experiment on identification of certain amino acids.. I will be highly gratified to have some valuable suggestions.. as I don't have an option due to lack of resources.. :(
Hi!! Its very nice of you to help me. TLC requires TLC tank/plate which wont be sponsored by them and I need to distinctly identify the essential amino acids. If there can be some substitute arrangement possible , then it will be perfect. :)
I agree with Krzysztof. Also if u just need to identify which aa's u have in your mixture, you can try to do the qualitative reactions for different aa's.
@Krzysztof Mine is the third one and I am deeply grateful for your help. I'll definitely try it out.. :) and about the institute I hate them..they awful people but I am stuck here so can't do anything other than carry out my work with whatever resources I have.. If I'll say anything to the administration they will probably ask me to stop my work and wait till I get enrolled in PhD. course somewhere.. :(
@Aleksandra i'll :)
why not resort to the old nice simple procedure of carrying even paper chromatography and stain with ninhydrin. Using standered amino acids to identify your mixture of amino acids
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