Which commercially available Pyrophosphate(PPi) Detection Kit works best for cellular level PPi detection?

07 August 2014 7,593 1 View

Can anyone please suggest if other than PBS and HEPES there is any other buffer that can be used for washing HeLa cells?

I have been conducting an experiment on HeLa cells; I do not need phosphate contamination or at least minimize phosphate interaction with my culture as much as I can. Thanks

05 June 2014 7,035 5 View

Can anyone explain in detail the difference between Ferric Citrate and Ferric Ammonium Citrate?

I was able to find that Ferric citrate provides iron in less toxic form than other free iron salts but was not able to find any detail on either how such mechanism is possible.

04 May 2012 6,350 0 View

Can anyone explain the difference between Bile Esculin Agar and Esculin Iron Agar used in Esculin Hydrolysis Test?

The composition of both the Chemicals is almost same except for the presence of Ferric Citrate in the former and Ferric ammonium citrate in the latter.

04 May 2012 7,644 0 View

How does increased NaCl concentration in culture broth effect the growth Vibrio Cholera?

Gram negative bacterial culture.

03 April 2012 7,892 22 View

Does anyone knows the complete protocol for arginine dihydrolase test done for Vibrio cholera identification?

I have found several links but none of them provide the whole experiment. There is one done by Thornley M.J. (1960) but I am not able to access it. Kindly help me out. :(

01 February 2012 6,386 2 View

Kindly help me with some protocols on spectrophotometric analysis of amino acids..As my institute doesn't provide me with HPLC and other equipments..

I need to conduct an experiment on identification of certain amino acids.. I will be highly gratified to have some valuable suggestions.. as I don't have an option due to lack of resources.. :(

31 December 2011 9,156 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View