What is the best method to dissolve curcumin in DMSO?

04 May 2016 4,898 1 View

Which commercially available Pyrophosphate(PPi) Detection Kit works best for cellular level PPi detection?

07 August 2014 7,593 1 View

Can anyone please suggest if other than PBS and HEPES there is any other buffer that can be used for washing HeLa cells?

I have been conducting an experiment on HeLa cells; I do not need phosphate contamination or at least minimize phosphate interaction with my culture as much as I can. Thanks

05 June 2014 7,035 5 View

Can anyone explain the difference between Bile Esculin Agar and Esculin Iron Agar used in Esculin Hydrolysis Test?

The composition of both the Chemicals is almost same except for the presence of Ferric Citrate in the former and Ferric ammonium citrate in the latter.

04 May 2012 7,644 0 View

How does increased NaCl concentration in culture broth effect the growth Vibrio Cholera?

Gram negative bacterial culture.

03 April 2012 7,892 22 View

Does anyone knows the complete protocol for arginine dihydrolase test done for Vibrio cholera identification?

I have found several links but none of them provide the whole experiment. There is one done by Thornley M.J. (1960) but I am not able to access it. Kindly help me out. :(

01 February 2012 6,386 2 View

Kindly help me with some protocols on spectrophotometric analysis of amino acids..As my institute doesn't provide me with HPLC and other equipments..

I need to conduct an experiment on identification of certain amino acids.. I will be highly gratified to have some valuable suggestions.. as I don't have an option due to lack of resources.. :(

31 December 2011 9,156 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Any stable dye to color acrylamide hydrogel beads?

Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...

14 January 2021 4,383 8 View

How to phosphorylate specific residues on pymol?

Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...

05 January 2021 230 3 View