Gunjan Pandey

8 Questions 11 Answers 0 Followers

Questions related from Gunjan Pandey

What is the best method to dissolve curcumin in DMSO?

I was not sure if we must heat the solution or is it readily dissolvable by vortexing only? Please let me know about the time and temperature if such a condition is required. Thanks in advance

05 May 2016 4,947 1 View

Which commercially available Pyrophosphate(PPi) Detection Kit works best for cellular level PPi detection?

I have to detect PPi levels in multiple cell (mammalian) lines.

08 August 2014 7,655 1 View

Can anyone please suggest if other than PBS and HEPES there is any other buffer that can be used for washing HeLa cells?

I have been conducting an experiment on HeLa cells; I do not need phosphate contamination or at least minimize phosphate interaction with my culture as much as I can. Thanks

06 June 2014 7,079 5 View

Can anyone explain the difference between Bile Esculin Agar and Esculin Iron Agar used in Esculin Hydrolysis Test?

The composition of both the Chemicals is almost same except for the presence of Ferric Citrate in the former and Ferric ammonium citrate in the latter.

05 May 2012 7,685 0 View

Can anyone explain in detail the difference between Ferric Citrate and Ferric Ammonium Citrate?

I was able to find that Ferric citrate provides iron in less toxic form than other free iron salts but was not able to find any detail on either how such mechanism is possible.

05 May 2012 6,396 0 View

How does increased NaCl concentration in culture broth effect the growth Vibrio Cholera?

Gram negative bacterial culture.

04 April 2012 7,926 22 View

Does anyone knows the complete protocol for arginine dihydrolase test done for Vibrio cholera identification?

I have found several links but none of them provide the whole experiment. There is one done by Thornley M.J. (1960) but I am not able to access it. Kindly help me out. :(

02 February 2012 6,436 2 View

Kindly help me with some protocols on spectrophotometric analysis of amino acids..As my institute doesn't provide me with HPLC and other equipments..

I need to conduct an experiment on identification of certain amino acids.. I will be highly gratified to have some valuable suggestions.. as I don't have an option due to lack of resources.. :(

01 January 2012 9,204 5 View