which commercialized BDNF antibody is good for western blot (mouse brain tissue)? .I bought one from SCBT, seems not good. I'm appreciated for your help.
Hi Haillong,
We recently released a BDNF polyclonal antibody that has been validated for use in western blot with mouse brain tissue. Here's a link to the product page:
Anti-BDNF Antibody (Catalog No. SPC-703)
http://www.stressmarq.com/products/antibodies/bdnf-antibody-spc-703/
We also offer an antibody trial program where you can try a sample size (12 ul) for free, you just pay for shipping. Here's the link to that page:
http://www.stressmarq.com/free-antibody-samples/
Best of luck with your experiments!
To detect pro-BDNF try anti-proBDNF from Millipore (rabbit polyclonal, AB5613P, Merck-Millipore). To detect mature BDNF, try anti-BDNF from Abcam (rabbit monoclonal, ab108319, Abcam). Good luck!
Has anybody observed 20-22 kdA BDNF band along with 14kda mBDNF, 28 kDA truncated BDNF and 32 kDA Pro-BDNF? What is this 20-22 kDA BDNF band?
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