21 Questions 80 Answers 0 Followers
Questions related from Apurva Agrawal
Hi, I am trying to look for basal ERK phosphorylation in N2a cells. Although, I am able to detect very good intensity of bands for total ERK, but the p-ERK bands are hardly visible. Also, when...
15 December 2017 974 4 View
Hi, I want to study drug- receptor interactions. Specifically, I would like to know to which receptor my drug of interest binds. Please help me with the methodology to do so... One method which I...
11 May 2015 1,337 1 View
I am interested in doing a meta analysis study based on already published literature especially using protein based data to test one of my hypothesis and bring out the signalling networks acting...
23 March 2015 2,727 2 View
I recently performed calcium imaging on some cells. I have exported out the time series data of all the cells in the field of view i.e. ~250 cells. Now I want to analyse the time- series calcium...
23 December 2014 6,813 25 View
I want to design a Live- cell Calcium Imaging experiment on some cells. I have four groups Control, Stimulant, Stimulant+ Drug/ Inhibitor, Inhibitor Alone. Should I perform experiments for each...
23 December 2014 7,785 11 View
Hi, I am trying to prepare PLGA based nanoparticles for hydrophilic drugs. I tried double emulsion solvent evaporation technique which resulted in only 0.5 % loading capacity and 20% entrapment....
05 August 2014 186 6 View
I am trying to find a suitable model to study certain questions. A. Dynamics of molecular subcellular localisation under different conditions. B. Calcium Dynamics. To study these two objectives...
15 July 2014 3,842 1 View
I am looking for methods by which I can knock down the expression a protein in acute hippocampal slices using siRNA. First of all, is it possible to do so using siRNA in acute hippocampal slices?...
07 May 2014 4,804 5 View
I am interested in understanding the spatial dynamics of certain proteins with time in live neurons. Instead of creating fusion proteins, I was thinking of using quantum dots based conjugation of...
07 May 2014 9,616 6 View
I am trying to design PLGA nanoparticles coated with Tween- 80 that will be carrying a hydrophilic drug. The PLGA NPs with the drug have been successfully made. But I am trying to figure out if...
02 May 2014 6,082 4 View
I have been trying to test the toxicity of Amyloid beta oligomers on neurons by measuring ROS. I am observing that the ROS response on exposure to these oligomers is highly variable when tested...
26 April 2014 6,193 17 View
I am trying to understand how depolarisation induced with KCl or glutamate/ NMDA or any such stimuli leads to the balance of actin polymerization: actin depolymerization in neurons. Is it possible...
21 October 2013 6,592 1 View
I am looking for methods which can be used to investigate whether the synapses which are being formed after some drug's treatment are actually forming functional synapses. Is the activity restored...
19 October 2013 7,055 9 View
I am looking for methods and targets on which one should look at if we are studying a molecule's role in maintenance of synapse for long- term after certain activity is induced. Let's say if it...
19 October 2013 2,673 0 View
I am working on a protein which becomes active when phosphorylated at a particular tyrosine site. I am looking for methods/ strategies as how to make constitutively active mutant for this protein....
18 October 2013 8,218 15 View
I am looking for methods to differentially activate synaptic and extrasynaptic NMDA receptors. There are reports where researchers have used Bicuculline, nifedipine and glycine to activate...
25 September 2013 3,355 4 View
I am currently facing problems with the generation of ROS in C8D1A astrocytes. There are inconsistencies with the results. The same when stimulated with h2o2 one time may give good ROS production,...
29 August 2013 9,756 0 View
I am looking for methods used for the visualisation of dendritic spines that can be further used for analysis. I recently came across papers which use DiI dye in finely powdered form to be applied...
23 August 2013 3,970 24 View
I have recently performed Thioflavin-S staining procedure to visualise amyloid plaques in the tissue sections of Alzheimer Disease mouse brain. Hence, I am looking for proper methods to quantitate...
25 January 2013 9,987 10 View
I am looking for specific literature to understand what happens with the calcium levels during early LTP phase and Late- LTP phase. It is very well known Calcium influx is necessary for LTP...
07 January 2013 3,952 1 View
I am currently working on preparing amyloid beta (1-42) oligomers and characterising them. The protocol that I use for the same is: 1. 1mg of amyloid pure peptide is dissolved in HFIP (400 ul)...
30 November 2012 1,698 58 View
