Which column and suppressor are most effective for separation of protein and enzyme using ion chromatography ?

More Ravi Kumar Sonwani's questions See All

Calculate the Strain at a Node in the Turbine Blade FE Model?

Hello Team, I would like to calculate the strain at a node #5 in a direction (l1,m1,n1) in the turbine blade FE model as shown. This node is connected with four elelemnts (#1,#2,#3,#4) and I have...

11 January 2021 7,702 3 View

One invention granted for the US and European patent will be counted as one patent or 2 patents for CV writing?

05 January 2021 8,135 4 View

Buckling of Smart composite plates using FEM ( MATLAB code)?

Can anyone help me with the FEM coding for Buckling of composite plates using the FSDT or HSDT - Source or the helping material (textbook, research paper etc) - Geometric stiffness matrix...

03 January 2021 3,059 3 View

How to optimize a structure for excited state when there is a bond breaking during optimization in gaussian?

In Gausssian, I have tried to reoptimize that structure by freezing the bond which is breaking in excited state during optimization. Before freezing the bond i have already tried scf = xqc and scf...

20 December 2020 8,444 3 View

How to build a polymer pdb file from a monomer pdb file, for using it is an initial configuration in Gromacs?

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28 October 2020 1,051 3 View

Need help in designing a countour which create a seperation bubble on a flat plate ?

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21 October 2020 6,635 5 View

Can someone suggest to me Fluorescence camera for intravital microscopy of blood vessels?

Can someone suggest to me a good and not so expensive Fluorescence camera for intravital microscopy of blood vessels? or can someone advise me on choosing the right camera for intravital...

15 October 2020 4,461 1 View

Moment curvature plot in SAP2000: What should i refer to understand the background calculation which SAP2000 does to generate the M-phi ?

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I am requesting you all to assist in the direction of epidemic diseases outbreak prediction using machine learning or deep learning approaches?

Any one who are in that track kindly give an idea of how to forward in this idea. Thanking you all

26 July 2020 2,898 3 View

How to extract electron and hole wave functions of a quantum well in Crosslight APSYS?

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19 June 2020 8,097 3 View

Waters 2707 HPLC autosampler problem?

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02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

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01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

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28 February 2021 6,477 1 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

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24 February 2021 2,602 1 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

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24 February 2021 5,974 4 View

Any easy method of analyzing vitamin D in foods by HPLC?

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24 February 2021 7,524 7 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

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23 February 2021 1,832 4 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

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21 February 2021 5,614 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

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21 February 2021 5,379 2 View

Adam B Shapiro

For ion exchange chromatography, you can use either an anion exchanger such as Q-Sepharose, or a cation exchanger such as SP-Sepharose.

Sephadex G50 is not an ion exchange resin; it is a gel filtration resin. This can also be used for protein purification. For gel filtration using and FPLC, you should use a Superdex column.

Steingrimur Stefansson

Hi Ravi, this a very general question and there is no "one-size-fits-all" answer.

I'm sure that if you scour Google Scholar you will find a purification procedure for your enzyme or a similar enzyme.