Which are the best methods to dye plastic microfibers in a sample?

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Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

04 August 2024 4,090 0 View

What are the best ways to the recount of microalgae?

I know the neubauer method, but I hear about the recount of growth with spectrophotometer.

23 June 2024 9,295 1 View

¿Cómo se implementaría una metodología participativa que involucre a personas mayores en el diseño y desarrollo de programas de bienestar Social?

Nos enfocamos en como diseñar estrategias participativas para involucrar a los mayores, y lo más importante es que respete su autonomía y dignidad. Buscamos entender como incluirlos en la toma de...

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How to get total dna solubilization?

I have a question, I have noticed that when I extract dna, in the last step when I resuspend in either 50 microliters of TE buffer or water, the total precipitated dna doesn`t solubilizes. I haved...

21 December 2023 161 1 View

Is it possible that phenolic compounds other than flavonoids are detected in the total flavonoid assay?

Hi, I would like to know how much specific is the TFA. It could takes into account not only flavonoids but also other phenolics compounds, such as tannins for example?.

06 November 2023 5,610 3 View

Does anyone here work with the luciferase detection system? I'm looking for a research center in Mexico that has a luminometer. ?

Hi I will be working with some constructions to detect relative luciferase activity but I need to know if anyone has worked on this, specifically with the type of detection kit and equipment...

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How to determine the sample size for a qualitative variable (Apt or not)?

We are designing the test where we will select a specific bacteria from different samples and we will seek to find this bacteria with the characteristics that we are looking for (genes), it will...

04 May 2023 4,212 1 View

Why can't I see a 20bp band in a native polyacrylamide electrophoresis?

I am doing PCR-RFLP to detect SNP, after the restriction enzyme digestion I check with 15% polyacrylamide gel, if the restriction occurs I should see two bands (126 and 20bp) but I only see the...

05 February 2023 1,432 4 View

ABAQUS Film condition for user-defined element?

I am trying to develop a UELMAT for a steady heat transfer analysis. My problem is that my element doesn't work when convection interaction is applied to it. I have tried both to apply this...

29 November 2022 4,178 1 View

Anybody else encounter PCR inhibitors when trying to extract and detect environmental (eDNA) from seawater samples?

Hi everyone, Would appreciate any help with this problem. I have established in-lab protocols that have seemed to work in extracted and detecting eDNA from water systems (we have fish systems...

16 October 2022 4,317 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Water Consumption in Plastic Recycling: Is it Higher Than for Virgin Plastics?

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29 July 2024 4,630 0 View

Waht is the effect of isoelectric point on MB dye photodegradation by ZnO nanoparticles?

Recently I have submitted an article regarding MB dye degradation by ZnO nanoparticle. I received a suggestion to include the discussion on the effect of pH. I want to know what is the impact of...

19 July 2024 4,868 1 View

Interpreting ATR-FTIR Spectra for Microplastics Research?

Hello everyone, I am currently pursuing a PhD in Environmental Engineering with a focus on microplastic studies. My background is in Civil Engineering, and I am relatively new to working with...

18 July 2024 7,258 3 View

Help me to identify these fungal slides?

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Why am I seeing only one initial maximum in torque-time curves for the fusion of PVC in a Brabender?

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How can magnetic Biochar remove dye pollutants, is this technology beneficial?

The harmful Dye pollution from industrial processes such as textile generated how to remove with magnetic biochar ?

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Sybergreen qpcr troublshoulting?

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What are possible reasons for having the repeated wavy patter in Raman spectrum?

I'm currently analyzing the Raman spectra of coloured plastic samples and have encountered a wavy, oscillating pattern in the spectra of some coloured samples. I was wondering what the possible...

04 July 2024 1,949 4 View

T. H. Al-Noor

https://pubs.rsc.org/en/content/articlehtml/2017/ay/c6ay02415g

Florenci González

I send you a validated analysis method for the semi-automated quantification of microplastics by using Nile red:

Article Lost, but found with Nile red; a novel method to detect and ...

Alethia Vazquez

Thanks for your help!