Which are the best methods to dye plastic microfibers in a sample?

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RTD Step analysis with matlab?

Hello to all my dear colleagues. I need to get my E(T) curve for my reactor. I already have the measures but I ignore how to procede with the data analysis. I have got Matlab but i am not sure if...

21 June 2020 2,675 5 View

Help about chemical sludge characterization?

Hello. I did run some coagulation / flocculation experiments[FS1] and I have been requested to characterize the sludge. I am ignorant on how to so. I was only requested to do SEM, XRD and Zata...

16 June 2020 8,715 7 View

In solar heterogeneous photocatalysis, is solar geometry relevant?

My project is supposed to considered solar irradiation as an important variable for heterogeneous photocatalysis. I haven't found any study that considers solar geometry as a variable of study in...

30 January 2020 744 3 View

Why do I have a Jagged line on my qPCR amplification?

I am performing a qPCR standardization of some MGB probes. I have tried just a few times but I don't want to waste reagents, every time I tried the assay, there is a jagging line. I am thinking...

13 November 2019 4,554 3 View

Do you have information about monoglycerides determination by Normal phase HPLC using a ELS detector?

We determine monoglycerides by normal phase HPLC using a ELS detector obtaining reliable results. Now we have a new equipment (SHIMADZU UFLC) with the same type of detector. However, when...

09 October 2019 1,244 3 View

Can a probe work with less Tm than the primers?

I found 2 interesting articles for the detection of bacteria at Phylum level with MGB probes, claiming that they work. I want to use that primers however when I use the oligoanalyzer tool, it...

08 July 2019 6,409 3 View

Protocol for polymer stainning with Ruthenium tetroxiode?

I cannot find a comprehensive protocol for stainning polymers with Ruthenium tetroxide solution. I would like to know the instructions and safety precautions for stainning and handling of...

04 November 2018 7,875 2 View

Does anyone has a protocol for measuring dopamine using and ELISA? . Does ELISA id DA levels or only TH or DAT?

Hi All, I'm completely new in the dopamine world and ELISA. I collected fresh brain samples and I have them in -80C. Do you have any protocol for ELISA brain sample preparation? I know the kits...

12 August 2018 1,811 3 View

Measurement soil pH in situ?

Somebody know how measurement the soil pH, according of mexican normative in laboratory I can use CaCl2 and I need wait 30 min, but I am thinking to determine soil pH in field with way faster that...

05 June 2018 8,337 2 View

I am trying to make siRNA transfection with Lipofectamine 2000. Can you give me some advice on how the most effective way to do this?

I am trying to perform an siRNA transfection with Lipofectamine 2000 into a 96 well plate, but I have some trouble preparing the reactives. I want a final concentration 50 nM of siRNA, but I found...

24 April 2018 2,734 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Droplet PCR in plastic chips always result an unexpected band, any suggestion of possible contaminations and how to improve?

I am doing droplet based digital PCR inside plastic chips. I can get the correct band, but I also consistently get an unexpected band, slightly shorter. I attached a typical gel image here....

18 February 2021 6,618 2 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View