Hi
I will be working with some constructions to detect relative luciferase activity but I need to know if anyone has worked on this, specifically with the type of detection kit and equipment available at a research center in Mexico.
Alethia
We are trying to identify microfibers in water samples
28 April 2019 2,297 3 View
I am using western blot to investigate the level of expression of the g protein, Gq, in cardiovascular, renal, and arterial tissues. I have been getting prominent bands at 42 kDa and 25 kDa. The...
18 December 2017 5,999 2 View
(Transfection with two plasmids has worked for this cell line. pRL-TK(Renilla) is not getting transfected).
16 June 2024 6,687 2 View
I am working on a project that requires stimulating RAW 264.7 NF-kB macrophage and readout my signal by luciferase assay. Although all concentrations tested (10, 50, 100ng/mL) after 24 hours...
16 June 2024 2,885 3 View
Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...
13 June 2024 9,936 5 View
I use raw 264.7 NF-kB cell line and the empty wells has a high absorbance as close as the blank well.
20 May 2024 1,518 4 View
I am a Master's student now and I am currently working on a project related to mRNA loading lipid nanoparticles and I want to check the translation ability of mRNA on HEK293T cell lines (I will...
23 April 2024 5,321 1 View
We have an immortalised human cell line that was modified using the PiggyBac transposase system to stably express luciferase. The transgene cassette contains luciferase-IRES-puro, the cells were...
27 February 2024 5,892 0 View
I am supposed to do luciferase assay for HBV enhancers EnhI and EnhII. I am quite new to luciferase assay and never done on hands before. So, I have to test certain protein effect on HBV enhancers...
07 December 2023 1,927 1 View
Hi all, I did a mock transfection using pGL3 vectors with different promoter inserts (plasmids range from 10 to 14kb). However, I have gotten unexpectedly low luminescence ratios (firefly luc /...
24 November 2023 746 3 View
is it necessary to starve cells while using a dual luciferase assay to detect expression on TGF-b cell signaling? I will perform an experiment and I would like to know the pros and cons of...
01 October 2023 6,463 2 View
Hello researchers, I am currently performing Dual-Luciferase assays in S. cerevisiae to study the response of a promoter to Mn2+. As a control, I integrated the expression cassette containing...
17 September 2023 3,353 1 View