Which are the best methods to dye plastic microfibers in a sample?

We are trying to identify microfibers in water samples

28 April 2019 2,162 3 View

Why am I obtaining non-specific bands for tissue samples?

I am using western blot to investigate the level of expression of the g protein, Gq, in cardiovascular, renal, and arterial tissues. I have been getting prominent bands at 42 kDa and 25 kDa. The...

18 December 2017 5,976 2 View

Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View

Luciferase assay without luminometer?

Hi everyone. I intend to carry out a luciferase assay for my research but have no access to a luminometer. Is there an alternative assay that I can use for my research or can anyone suggest a way...

05 December 2020 9,749 2 View

What is the maximum passage number of SKOV-3 and SKOV-3-Luc cells?

Hello, for my experiment I'm working with SKOV-3-Luc cells, which are SKOV-3 cells stably/continuously expressing the firefly luciferase enzyme. These cells are obtained by the transfection of...

25 November 2020 3,219 3 View

Luciferase assay for gene-miRNA interaction: which vectors should be used?

23 November 2020 2,813 2 View

Does anyone have experience in TOPFLASH assay in HEK293T cells?

I did lipofectamine transfection in 12-well plates with 0.5M of cells and 0.8ug of TOPFLASH plasmid in each well. After 24h, I changed to media to serum-free media supplemented with 10uM...

20 October 2020 3,584 2 View

Luciferase assay for miRNA-gene: How to construct the mutated prime?

I have experimental evidence (qRT-PCR and WB) to suggest a miRNA downregulates a gene. To test if the regulation is direct I want to perform a luciferase reporter assay. So I designed a primer...

15 October 2020 7,641 4 View

Will Cypridina Luciferase (CLuc) Catalyze Beetle Luciferin?

Hello, I am using a lentivirus encoding CLuc to transduce target cells. I want to then lyse the cells in the presence of luciferin and measure luminescence. The kit which I had hoped to use...

15 September 2020 6,664 2 View

How to perform cell transfection and Dual Glo assay with low-adhesive (HEK293) cells?

Hello everyone. I need to use Promega Dual Glo assay for HEK293 cells, but I am really afraid of wash out my cells from the 96-well plate, and at the same time, I am afraid to use coating because...

09 September 2020 4,817 7 View

Detecting Luciferase by Western blot?

My colleague is trying to detect Luciferase by Western blot and what she got is nonspecific binding - figure A (?). On fig.B she was using anti-His Tag antibody just to check if the sistem is...

04 August 2020 2,533 1 View

How best can you measure primary hepatocyte viability after they are plated on collagen plates?

We are currently trying to create a hepatocyte clearance method that can be used for compounds which are slowly cleared. To do this we are plating the hepatocytes on collagen 48-well plates...

19 March 2020 9,825 2 View