Which anti-CTB (cholera toxin subunit B) antibody is good for IHC (hamster brain tissue)?

More Brooke Nichole Dulka's questions See All

ANY-MAZE 7.09 Crashing when I add a wmv file?

Whenever I try to add .wmv files as a video source that have worked in previous versions of ANY-MAZE, the new version 7.09 crashes with error code 0xC0000094 at 0x4186d19F. Has anyone experienced...

07 January 2022 9,682 1 View

Difference between peptone and proteose peptone for culture medium?

Hi! I am looking to make a medium for culturing fungi. I plan to make DCPA (Andrews and Pitt, 1986 Selective medium for isolation of Fusarium species and demat... ) and I am wondering if I can use...

06 September 2021 8,456 7 View

Can anyone share an article specifically on telehealth theory development and/or applications?

Hello, Can anyone share an article specifically on telehealth theory development and/or applications? Thank you! Best, Brooke

15 June 2021 5,001 7 View

Why is my cryostat taking extra thick sections?

I have a problem with sectioning brains on a Leica cyrostat. We are trying to take 20 um coronal sections (which we have had no problem doing in the past) but it looks like the cryostat is taking...

03 November 2019 5,870 2 View

Is there a minimum diameter required for the formation of a lipid bilayer in secreted vesicles?

Hoping to give context to non-membrane limited vs. limited membrane vesicle formation and subsequent size distributions of these secreted vesicles. An example of this would be with exomere...

27 August 2019 4,334 1 View

Is there a way of running cluster analysis with missing data?

Hello Everyone! I am trying to run cluster analysis on a set of large mammal data in order to try and recreate ecological profiles for extinct species. However, as one would expect with these data...

19 March 2019 7,836 8 View

How do pregnancy contractions effect blood glucose levels in a type 1 diabetic?

I am 33 weeks pregnant and a type 1 diabetic. I have been told by doctor's that I am already starting to have contractions but no dilation or indications of delivering soon. Since the contractions...

01 November 2018 926 4 View

why can't i delete this old question?

...

18 September 2018 1,778 5 View

Reduction of graphene oxide coated sample with sodium borohydride?

I have a metal coated sample with a graphene oxide film and want to organically reduce it to rGO. If I'm using sodium borohydride, do I simply make the solution using DI water? I know it has a...

06 July 2018 3,934 5 View

What statistical test to use: dependent variable is binary and independent variable is continuous?

Just wondering if anyone can save me another 8 hours of headache trying to figure out what stats to run on my data.. I am trying to understand whether a number of different factors, such as age...

10 May 2018 8,874 8 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Russell Linscott

Do you mean cytotrophoblastic cells or some other CTB??

Brooke Nichole Dulka

cholera toxin subunit B

That makes more sense ;-). Not a specific recommendation, but here are some sources for anti-cholera toxin B you may want to explore. You can filter for IHC application:

https://www.linscottsdirectory.com/antibodies?q=cholera%20toxin%20b

Hope this helps.