Brooke Nichole Dulka

5 Questions 10 Answers 0 Followers

Questions related from Brooke Nichole Dulka

Why is my cryostat taking extra thick sections?

I have a problem with sectioning brains on a Leica cyrostat. We are trying to take 20 um coronal sections (which we have had no problem doing in the past) but it looks like the cryostat is taking...

04 November 2019 5,912 2 View

Which AAV sterotype gives best local transfection in rat hippocampus?

I'm looking to transfect rat hippocampal neurons (and possibly amygdala) with an AAV vector but I am unsure which AAV serotype to use. I want good local transfection and minimal...

11 September 2018 3,752 6 View

Should I store my CNO (clozapine n-oxide) stock solution at room temp or -20?

CNO (clozapine n-oxide) is the ligand for DREADD experiments. I have made a stock solution but am not sure what temperature to store it at.

21 July 2017 5,643 2 View

Which anti-CTB (cholera toxin subunit B) antibody is good for IHC (hamster brain tissue)?

Double labeling c-Fos + CTB cells in the infralimbic cortex

20 June 2017 8,899 3 View

What works and what doesn't with social defeat and female C57 mice ?

I want to use social defeat to stress female C57/BL6 mice. Has anyone had any success doing this? What works and what doesn't?

29 June 2015 7,305 3 View