Which among the following cell lines is best to be used for titrating anti-mouse EpCAM antibody by Flow cytometry?
1. Caco-2
2. DLD-1
3. HCT-116
4. SW480
should i dissolve it in DMSO or acetonitrile for stock solution; its aqueous solution cant be stored for more than one day. would it be compatible to mice.
21 August 2022 8,600 0 View
how can i measure the resistivity of the mgo nanowires deposited on the silicon substrate?
08 June 2022 6,527 2 View
i wnt to deposit Au or Al electrode on my silsicon substrate deposited with nanowires can any one help me how i can do this by myself the companies iam approaching are very expensive ..
11 May 2022 952 8 View
i need to know the exact step size and scan speed for the MgO nanowires xrd deposited on the silicon susbtrate whenever i do xrd it just shows silicon peak and MgO peaks gets vanished at the end...
28 March 2022 3,751 4 View
Is it possible to share data on mosquito species diversity among countries with land boundaries? The expected result is a revision to the mosquito species checklist between corresponding...
19 January 2022 743 4 View
Wing geometry analysis usually involves wing detachment to avoid capture bias. I am curious if analysis of wing geometry can be done without detaching the wings. This is sometimes important when...
19 January 2022 386 1 View
i want to etch the MgO nanowires from the silicon substrate deposited with cvd i need to transfer the naonwires to the glass subastrate to deposit electrode anyone can help please ?
14 December 2021 7,711 7 View
The samples were preserved at -80C, minced with sterile surgical blades in trizol (on ice) and proceeded with usual steps of Trizol extraction. Initially the pellet obtained was clear, easily...
24 September 2021 527 2 View
I have 278 nucleotide sequences of a pest species. I am trying to build an ML phylogenetic tree by BEAST using CIPRES portal. I have converted fasta file into .xml using BEAUti. Then I used that...
30 August 2019 8,444 12 View
Can we use the same medium for co-culturing intestinal epithelial cells snd immune cells? If yes what should be the composition of culture medium?
07 August 2017 2,480 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View