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Questions related from Tabasum Sidiq
Can we use the same medium for co-culturing intestinal epithelial cells snd immune cells? If yes what should be the composition of culture medium?
08 August 2017 2,494 1 View
I want to check the activation status of CD8+ T cells from mouse spleen. I was wondering which activation markers I should use. Will CD44 and CD62 L together work to differentiate the naive CD8+ T...
20 July 2016 2,000 4 View
I wanted to know which marker I should use to sort the splenic macrophages from mice by flow cytometry? Actually I checked the literature and observed that people use CD11b or F4/80 as the marker...
13 June 2016 5,016 3 View
Can anybody share the protocol for isolating microglia from mice brain as I need to sort the isolated microglia for RNA-Seq purpose.
25 December 2015 1,443 5 View
I am trying to isolate total RNA from FACS sorted CD4 T cells for RNA-Seq purpose. However, I get the low yield of RNA (about 1.5 ug) from 1-2.5 million FACS sorted CD4 T cells. Since I need upto...
17 August 2015 6,628 12 View
Has anybody in this group quantified the bacteria (Firmicutes, Bacteroides) in the ileum and feces of mice using the plasmids having 16s rRNA for respective groups cloned inside. I am getting the...
22 June 2015 6,928 2 View
I need to isolate brain lymphocytes from mouse. The protocols I read are mentioning of using RPMI or HBSS without phenol red. I have both of these reagents having phenol red in them. Is there any...
29 May 2015 1,020 3 View
Hi, I have to analyze iTreg and nTreg cell populations from the spleen of mice by FACS. I have selected the fluorochrome combination as: FITC Helios PE Foxp3 PerCP Cy5.5 CD127 PE-Cy7 CD304 APC...
14 May 2015 6,791 0 View
I am doing mixed lymphocyte reaction for testing the reaction of C57BL/6 splenocytes to Balb/c splenocytes. I cultured the whole splenocytes of Balb/c mice with the irradiated splenocytes of...
08 May 2015 8,788 5 View
I need to stimulate CD8 T cells to check their proliferation by CFSE staining. Is it a good idea to add the soluble CD3 antibody to the co-culture of CD8 T cells and irradiated APCs to check the...
18 February 2015 7,275 12 View
I am facing the problem in culturing H.hepaticus on 5% Trypt Soy Agar plates. I successfully cultured it in the past. Using the same strategy, now I am not able to trouble shoot the problem even...
25 January 2015 9,247 4 View
Which among the following cell lines is best to be used for titrating anti-mouse EpCAM antibody by Flow cytometry? 1. Caco-22. DLD-13. HCT-1164. SW480
19 November 2014 6,355 0 View
Can I use any other cell type other than TE-71 (mouse thymic epithelial cell line) to titrate EpCam (CD326) antibody for Flow cytometry?
30 September 2014 8,223 2 View
Can somebody suggest which murine cells should be used for titrating CD107 (LAMP1) antibodies for Flow Cytometry? My idea is that we can use irradiated splenocytes as APCs and stimulate these...
20 September 2014 7,627 11 View
Flow Cytometry
13 September 2014 4,182 11 View
Murine thymic epithelial cell isolation.
10 September 2014 9,929 3 View
Has anybody irradiated C57/BL6 mice at 400 Rad for adoptive transfer of EAE? In the first attempt, I irradiated C57/BL6 mice at 350 Rad and I could not observe successful induction of disease...
06 September 2014 4,410 13 View
I usually get two populations of cells, with two peaks in histograms whenever I stain mice splenocytes with this antibody. Can anybody share his experience with me if using this antibody?
24 August 2014 5,376 7 View
