Tabasum Sidiq

13 Questions 35 Answers 0 Followers

Questions related from Tabasum Sidiq

Can we use the same medium for co-culturing intestinal epithelial cells and immune cells in mouse?

Can we use the same medium for co-culturing intestinal epithelial cells snd immune cells? If yes what should be the composition of culture medium?

08 August 2017 2,448 1 View

What are the best markers to differentiate between naive and activated CD8+ T cells in mice?

I want to check the activation status of CD8+ T cells from mouse spleen. I was wondering which activation markers I should use. Will CD44 and CD62 L together work to differentiate the naive CD8+ T...

20 July 2016 1,968 4 View

Which is the best marker to sort splenic macrophages from mice by flow cytometry?

I wanted to know which marker I should use to sort the splenic macrophages from mice by flow cytometry? Actually I checked the literature and observed that people use CD11b or F4/80 as the marker...

13 June 2016 4,965 3 View

Can we sort CD4 T cells by MACS beads followed by their sorting by FACS for RNA-Seq experiments?

I am trying to isolate total RNA from FACS sorted CD4 T cells for RNA-Seq purpose. However, I get the low yield of RNA (about 1.5 ug) from 1-2.5 million FACS sorted CD4 T cells. Since I need upto...

17 August 2015 6,594 12 View

Can anyone help with bacteria quantification using plasmids for respective group?

Has anybody in this group quantified the bacteria (Firmicutes, Bacteroides) in the ileum and feces of mice using the plasmids having 16s rRNA for respective groups cloned inside. I am getting the...

22 June 2015 6,895 2 View

Is CFSE more sensitive method than MTT assay to detect proliferation of cells?

I am doing mixed lymphocyte reaction for testing the reaction of C57BL/6 splenocytes to Balb/c splenocytes. I cultured the whole splenocytes of Balb/c mice with the irradiated splenocytes of...

08 May 2015 8,737 5 View

Does soluble CD3 work as signal for T cell stimulation?

I need to stimulate CD8 T cells to check their proliferation by CFSE staining. Is it a good idea to add the soluble CD3 antibody to the co-culture of CD8 T cells and irradiated APCs to check the...

18 February 2015 7,245 12 View

Does anybody have experience in culturing H. hepaticus?

I am facing the problem in culturing H.hepaticus on 5% Trypt Soy Agar plates. I successfully cultured it in the past. Using the same strategy, now I am not able to trouble shoot the problem even...

25 January 2015 9,224 4 View

Which among the following cell lines is best to be used for titrating anti-mouse EpCAM antibody?

Which among the following cell lines is best to be used for titrating anti-mouse EpCAM antibody by Flow cytometry? 1. Caco-22. DLD-13. HCT-1164. SW480

19 November 2014 6,320 0 View

Can somebody suggest which murine cells should be used for titrating CD107 (LAMP1) antibodies for Flow Cytometry?

Can somebody suggest which murine cells should be used for titrating CD107 (LAMP1) antibodies for Flow Cytometry? My idea is that we can use irradiated splenocytes as APCs and stimulate these...

20 September 2014 7,591 11 View

What combinations of fluorochromes are to be used for 8-color flow cytometry so that there is no overlapping?

Flow Cytometry

13 September 2014 4,141 11 View

Can anybody provide me with the protocol for the isolation and sorting of murine thymic epithelial cells?

Murine thymic epithelial cell isolation.

10 September 2014 9,885 3 View

Does anybody in this group use F 4/80 antibody for staining splenic macrophages by flow cytometry?

I usually get two populations of cells, with two peaks in histograms whenever I stain mice splenocytes with this antibody. Can anybody share his experience with me if using this antibody?

24 August 2014 5,331 7 View