Hi,
I was wondering what is the best way to preserve IBA, TDZ and NAA once in solution.
Thank you
Dear Nico,
I store IBA and NAA stocks at 4°C.
Good luck.
Dear Nico
Generally at 4°C but we also kept 1.5ml alliquot in 2ml tubes at -20°C and it worked without any problem if you dont thaw them frequently.
Ideally at -20 ° C and in small aliquots (1mL) in Eppendorf tubes.
Hello, So I meet a case of a person that in Europe has gluten sensitivity but never got positive in the celiac tests. When travelling outside Europe (Asia, Africa, Cuba) can eat breat and other...
09 October 2018 10,152 1 View
Hi folks, I was wondering what determines a protein to go into the regulated vesicular exocytosis. I am also interested in understanding how vesicles from the regulated pathway are arrested in...
05 June 2018 7,704 0 View
Dear Research gate community, I was wondering if any of you add a pH indicator and/or antioxidant to the culture agar media to produce explants. Thank you Nico
01 January 1970 7,674 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View