Antioxidant and pH indicators in your micropropagation media

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Ricardo Julian Licea-Moreno

Why you would need to add a pH indicator to culture medium? Regarding the antioxidants, several substances are used for: ascorbic and citric acids, activared charcoal.....

Nico Ortiz

Thanks for the reply. Is there no change in pH along the use of the media? Or an indicator of carbon source depletion.

Of course, important changes in pH of culture media might occur during the culture. I don't have references about indicators for carbon source depletion, although, great changes also occurr in mixotrophic cultures.