Where can we purchase the embryonic and adult rat whole brain cDNA in the UK?

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Differences in DNA fragment size between inputs and ChIPed DNA?

Dear all, We are noticing differences in DNA fragment sizes between input and ChIPed DNA in a ChIP seq experiment (e.g., Tapstation, BioAnalyzer data). We tend to see smaller fragments in the...

16 February 2024 3,567 0 View

Is it necessary that the I-V between source and drain should pass from (0,0) at any gate voltage?

I have fabricated MESFET by depositing GeSe thin film on glass substrate and then deposited silver metal for gate. Now when I record I-V between source and drain at 0 gate voltage it passes from...

19 October 2022 6,069 4 View

Can anyone suggest any kits or reagents which may help me in removing dsRNA contaminants after In-vitro Transcription (IVT reaction)?

I am generating mRNAs for intra-tumor injections in mice. I want to reduce non-specific immunogenicity due to dsRNA contaminants after IVT. Any simple protocols or kits which could ensure...

04 July 2022 5,817 1 View

Increase in lot size Consideration ?

Hi, we are trying to increase our lot size from 30,000 units to 72,000 units for one of our class IIb Medical device. From Quality standpoint, I am considering the change in sampling size with the...

22 February 2022 3,008 3 View

Is it possible to get direct bandgap greater than indirect bandgap?

I have synthesized Sb incorporated GeSe ternary alloys. The proportion of Sb varies as 10% and 15% by weight proportion. In 15% Sb incorporated GeSe, I am getting direct bandgap to be 0.94eV and...

08 December 2021 9,107 0 View

How to know the reaction site of Metal(II) ion i.e., Hg+2 ion in schiff base ligand by using gaussian?

Iam trying to know the reaction site of Hg+2 ion in schiff base ligand by using gaussian 09w. Please help me with this question. I am attaching the schiff base ligand gaussian file.

15 November 2021 9,919 2 View

How to perform modal analysis of pre-tension 4-strut simplex tensegrity structure in Abaqus?

Hi guys, I am trying to perform a modal analysis of a pre-tension 2D array of 4-strut simplex tensegrity plate in Abaqus. Can anyone tell me how to apply pre-tension to the struts and cables for...

07 November 2021 4,427 2 View

How to get the graph of shear stress and normal stress in PFC 2D?

I'm performing a direct shear test in PFC2D. I'm able to get the graph of normal stress vs no. of steps and shear stress vs no. of steps. But, I'm finding it difficult to get normal stress vs...

28 October 2021 8,844 3 View

Why Sb peak is missing in EDS spectra, whereas it is recorded in elemental mapping?

I have recorded EDS spectrum of Sb doped GeSe ternary alloy. Now my problem is, I am not getting any peak for Sb in the spectrum but when elemental mapping is conducted on the sample, a uniform...

31 August 2021 1,396 4 View

Normal trend of Raman peaks is the intensity increases with decrease in temperature. But is the reverse behavior possible?

In temperature dependent Raman studies the general trend of peak intensity w.r.t. temperature is that it decreases and starts broadening with increase in temperature. But in one of my sample I am...

21 May 2021 6,986 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?

29 July 2024 7,714 4 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

Ternary in Electrical Engineering?

Dear Research Community I would like to invite Elctrical Engineering specialists to solve problems related to Ternary Algebra or tripple sets I have an algorithm that places in relation three...

28 July 2024 5,771 2 View