Where can I purchase BON cell Line?

More Maher Elsheikh's questions See All

The best procedure to digest (by acid) NiFe/C powdered samples for atomic absorption?

Dear All, I would like to ask about the best way to digest NiFe/C powdered samples by acid for atomic absorption spectroscopy? Is HNO3 or HCL alone sufficient? Or is it better to mix them and...

23 April 2024 5,179 0 View

Hi, What is this organism?

Hi, What is this organism? I found it in Quaternary sediments!

25 March 2024 8,298 7 View

What is a systematic review study? What is the difference between a systematic review and a literature review?

I must write a systematic review study. Who is better and accepted in scientific journals?

18 January 2024 144 2 View

Is there any laboratory in Egypt for measuring the electrical switching properties of zinc oxide thin film?

Resistive random access memory

31 October 2023 9,851 0 View

Graphite exfoliation to graphene?

Hello, What are the the best methods (in terms of time and complexity) to exfoliate graphite to synthesize graphene? I have tried to do it using DMF as a solvent aplplying sonication followed by...

09 October 2023 3,780 3 View

How should the affiliation section be formatted for a student in an online Master's program when submitting a research paper?

Dear Research Community, As a newcomer to the field of research, I am seeking guidance on a specific matter related to affiliations in research papers. Consider a scenario where a student is...

21 September 2023 3,584 3 View

Why do l keep getting joint shear failure instead of beam flexural in ABAQUS modeling RC beam column joint?

Hello everyone, I am trying to make a model using ABAQUS for RC beam column joint, but I keep getting joint shear failure despite using 3 stirrups (D=8mm) inside the joint core. Given that, the...

18 September 2023 8,889 2 View

How to solve a problem in creating a model on Abaqus under two steps of loading?

I am a master student working on a thesis on the topic of retrofitting of reinforced concrete beam column joints, but I have a small problem if you may help me with it. I need to create a model on...

23 December 2022 9,753 2 View

Performing a gDNA extraction using the NEB Monarch gDNA Extraction from Tissue Kit. Can you see the DNA bound to beads?

I'm using the NEB gDNA extraction from tissue kit to get phage DNA as they have developed a method for doing so. I'm struggling with the step in the image below. There is this very viscous/stringy...

17 November 2022 4,970 0 View

Can anyone suggests for an industrial process using the propane as feedstock for it ?

Please, I need to design for an industrial petrochemical plant using a high purity liquefied propane as feedstock for that plant and the product/s is/are valuable and has marketing value rather...

11 October 2022 1,190 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Omer Faruk Kuzu

It seems that the only way is to ask a lab that has published a paper with this cell line.

Maher Elsheikh

Found them. Thank you for your response.

Alessandro Cinti

Hi Maher,

I'm looking for the same cells, where did you found them?

We got them from Dr. Helen Raybould at UC Davis.

[email protected]