Could someone help with interpreting Ag XRD peaks?

Dear All, I have prepared trimetallic PdAgNi nanoparticles supported on carbon in addition to their monometallic Pd/C and Ag/C counterparts. I am having some issues with the XRD pattern with the...

23 December 2020 7,986 3 View

What is a good TEM magnification to enable accurate small-size nanoparticle calculation?

Dear Fellows, I synthesize intermetallic nanoparticles by chemical reduction and disperse them on carbon support. The TEM equipment I use is unable to highly magnify above 40kx. Beyond that the...

23 September 2020 8,910 8 View

How to improve the SEM graph quality obtained by basic teaching SEM equipment?

Dear All, Could you please help me with improving the quality of my SEM images? An example image is attached. It has obtained by a basic Jeol SEM microscope with a tungsten filament. I have tried...

01 August 2020 9,903 6 View

What is the suitable reclamation and management for Shallow soils?

Shallow soils have less than 50 cm depth of solum. Generally, they have a thin A horizon over the bedrock or the parent material. Shallow soils are common on hilltops, hillsides, and throughout...

31 March 2020 8,821 7 View

Where I can find Cold Isostatic pressing facility in London or UK ?

I'm looking for Cold isostatic pressing machine in UK

11 October 2019 1,847 2 View

What are the degrees of freedom in statistics?

Degrees of freedom have a very important role in calculating the ANOVA table, what is the meaning of df, and why calculated n-1?

27 September 2019 5,266 3 View

Please, could someone explain the ORR mechanism to me?

Dear All, I prepare trimetallic Pd-based catalysts supported on carbon and apply them as electrocatalysts for ethanol electrooxidation which is my main target. Yet, an idea occurred to me that...

19 March 2019 7,280 3 View

For predicting future land use change, how to identify the end point of prediction time if the starting point is 2010? Is there any standard ?

I saw many studies have been done to predict future land use change around the world. Choosing the starting points of prediction time relies on the updated land use map. However, there is no...

16 October 2017 504 3 View

What are differences between retention, fixation, sorption, and adsorption in soil chemistry?

The terminology like retention, fixation, sorption, and adsorption are used in soil chemistry and material sciences. These terminologies always confusing. For example, the using this term in P...

12 October 2017 9,589 4 View

What is the suitable reclamation and management for alkaline soils

25 June 2017 1,595 10 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View