I am looking for a single place where I can get the detailed protocols for different kinds of cell for neurons related tissue engineering and regeneration. Please share links if you have any information.
Thanks in advance
raj kumar
There is a book name "Current Protocals Of Neuroscience"
onlinelibrary.wiley.com/book/10.1002/0471142301/homepage/archive.htm
onlinelibrary.wiley.com › ... › Current Protocols in Neuroscience
https://www.ncbi.nlm.nih.gov/labs/journals/curr-protoc-neurosci/
www.sciencedirect.com/science/book/9780128014615
You Can see which link is open.
This one has a lot of useful protocols: http://cshprotocols.cshlp.org/
Hope this will help.
Dear Raj,
There is also a book of protocols that has lots of helpful advice for culturing different types of neurons:
Culturing Nerve Cells, 2nd Edition. Edited by Gary Banker and Kimberly Goslin.
http://cognet.mit.edu/book/culturing-nerve-cells
Good Luck!
Jill
I am interested to conduct the drug release in different release media, but i would like to know is any restriction or limitation of the concentration of other components such as...
08 September 2019 7,406 3 View
I am looking for isomerism of steroids, can you share details, books, articles, reviews discussed on steroids isomerism in details Thanks Raj Kumar
08 September 2019 3,645 3 View
Hi, We have formulated drug nanoparticles. interestingly, we have not found the complete release of the drugs, however, the drug release and drug left in release media is less than the amount of...
08 September 2019 1,961 5 View
I am everyday finding very much excellent articles related to my work in research gate, but i am unable to save them for my future requirement. is it any possibility or option to make our own...
02 March 2019 9,574 4 View
Hi, I am looking for best papers on solid lipid nanoparticles which discuss science properly and interpretation of in depth results and discussion. it will helpful for me how to improve my...
02 March 2019 9,292 5 View
If we took permission for reproduction before submission of journal X, but if it is rejected from Journal X, then can we use same permission of reproduction in submission of any other journal Y,...
08 September 2018 3,567 7 View
I am working on magnetic nanoparticles coating with silica. but most of my experiments failed, Then I tried Au, Ag nanoparticles coating with silica. still facing issues. If you directly worked on...
07 August 2018 2,899 4 View
I am keep on performing experiments but instead of coated nanoparticles, I am getting separate nanoparticles of silica or gold or silver when i performed experiments are well reported journal. For...
06 July 2018 9,322 3 View
I found the chemical structure or formula of both are same even CAS No. But I want to know why both are different and why ?
05 June 2018 9,628 10 View
I am trying to prepare silica nanoparticles. I followed the literature as suggested that in all reagent in ethanol mixture added TEOS, in some other literature they added Ammonia solution to...
11 December 2017 3,408 3 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...
27 February 2021 6,350 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...
24 February 2021 9,210 1 View
I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...
24 February 2021 3,623 3 View