Where can I get a survey/questionnaire about employment related experiences/concerns of Persons with HIV?

More Donnamar Cameron's questions See All

How to calculate the orbital occupancy of Partial Density of States in VASP?

Hi, I have integrated the total DOS of a system up to the Fermi Energy and got the expected valence electrons. But when integrating the Partial DOS (PDOS) for each atom and summing these, the...

03 December 2019 2,714 3 View

Why my restiction enzyme treated chromasomal DNA has Plasmid results?

I conducted a practical whereby previously isolated plasmid and chromasomal DNAs that had been electrophoriesd in sepeerate gells where then both treated with the same restiction enzyme (EcoR1)...

11 September 2019 8,730 3 View

Where is my NTC contamination coming from?

I am currently performing qPCR and in the process of setting up relative standard curves to assess efficiency. I keep getting amplification in my NTC wells despite using new water and new SYBER...

13 August 2019 6,870 9 View

Intracellular dyes in patch pipette - cheap alternative to Alexa?

I generally include Alexa 488 hydrazide intracellular dye in my patch pipette to have a look at the morphology of the cell while I'm recording from it. It's getting a bit expensive now though,...

20 June 2019 1,464 3 View

Is there a published protocol for extracting lipids from skeletal muscle?

Hello, I am wanting to express mouse skeletal muscle contractile performance to lean tissue mass, and I had been advised in a piece of peer review for a paper that it is possible to perform such...

17 March 2019 716 1 View

Does anyone have any ideas for my research paper ? My topic is Steve Jobs ?

I need help on getting something to write about Steve Jobs. for my CIS term paper

17 September 2018 6,536 2 View

Whats the best way to extract and save venom from a live lionfish?

I'd like to pull venom from my pet lionfish. I can imagine that pressure and puncture of a basic seal on a beaker or something will work, but is there a good type of seal to use? and how should I...

21 March 2017 9,231 5 View

With the issue of seasonal tourism, what strategies can be put in place to combat this and spread tourism out through the year?

Seasonality at tourism destinations is a problematic issue as during the low months, resources aren't used and destinations cannot generate enough revenue. How can this be combatted in order to...

05 March 2017 1,631 3 View

Any advice on the vapor pressure behavior of Rose Bengal: outgassing or volatilization behavior?

I am using Rose Bengal as a photosensitizer coated on a substrate. To cure this layer we are considering use of a vacuum oven to remove the solvents but are concerned about outgassing of the RB....

08 October 2015 8,154 2 View

Where do I acquire zinc deficient soil?

Final Update: I've been in contact with the USDA & A&M's Ag Extension and they are in the process of combing through a recent soil survey to find soil that meets my specific needs. I ended...

30 November 2014 1,134 10 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

Is anyone else having a similar situation?

I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...

10 February 2021 658 5 View

What is the best concentration of Pam3CSK4 to coat a dish to activate with THP-1?

Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...

21 January 2021 2,593 1 View

Can I use 24 wells black tissue culture uncoated plate for cultuing macrophage (THP-1 treated with PMA) ?or I have to coat them?

Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...

05 January 2021 4,214 3 View

How can I change the media for THP-1 cells without dislodging them from the surface of my well plate?

I am working with THP-1 cells at the moment and am having trouble with maintaining a high confluence once I seed them on a 24-well plate. I usually try to seed around 100,000 cells per well and...

29 December 2020 1,567 3 View

Can HIV drugs will be a provisional or recurring option against COVID-19?

22 December 2020 7,872 2 View

Can the formation of drugs against coronavirus also be useful against the Influenzas virus or HIV?

Can the formation of drugs against coronavirus also be useful against the Influenzas virus or HIV? This is because these viruses shared many common proteases for viral replication.

22 December 2020 6,508 6 View

Is there any chemical or surgical method to induce hypoxia in mouse model?

Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...

15 December 2020 866 6 View

Does anyone have experience in infecting suspension cells (like THP-1 or K562 etc) by retrovirus?

I am trying to make stably expressing cell line of my gene of interest in Suspension cell line using retrovirus but i don't have recommended protocol. I have searched literature but mostly they...

14 December 2020 8,274 3 View

Emmanuel Ifeanyi Obeagu

Dear you can design the questionnaire and administer to the patient and get thier response in connection to employment.

Joyce P Yang

It might be useful to consult existing Stigma scales (e.g.:Article Measuring Stigma in People with HIV: Psychometric Assessment...

) and extract the items related to employment