Where can I get a survey/questionnaire about employment related experiences/concerns of Persons with HIV?

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Need help with a theory/principle?

My research question is How did the WBC ( World Baseball Classic) make baseball relevant to other countries? I need help choosing a theory or principle to frame my study.

04 September 2023 4,203 1 View

Systemic lactate resulting from muscle activity during exercise enters the gastrointestinal lumen to be metabolized by Veillonella?

I was left with this question after reading about increased aerobic capacity conferred via fecal/microbiome replacement after exercise in humans (endurance athletes) and mice. Can we conclude the...

02 May 2023 1,874 1 View

How to compare pollinator communities between two related plants?

Hello I am investigating the shared pollinator communities of a native wildflower, and a related invasive hybrid. The hybrid is replacing the native plant. I have collected data which lists all...

27 August 2022 9,283 8 View

"Keyword cards for step dependent input must appear after the first *step card. Analysis Input File Processor exited with an error." How to fix?

When trying to submit my model in Abaqus I get the error as shown above. I am not writing the code myself so am not able to re-order any keywords and am unsure how to fix this issue. I have had it...

22 August 2022 5,717 3 View

Is it possible to chlorinate SiO2 through reflux?

I have been working on acidified silicas and found that bare mesoporous SiO2, when refluxed in 12M HCl, is apparently very active in the reaction I'm looking at. With further testing on the...

13 April 2022 7,135 3 View

Who supplies Viton Glove Box Gloves?

I'm looking for Viton gloves for 8" ports on a glove box for handling aromatic hydrocarbons. Does anyone know of suppliers that still carry them? They seem to of been discontinued by Honeywell.

07 November 2021 8,042 3 View

What adapters are available for connecting tubing to glass capillary needle?

What adapters are available for connecting 1 mm OD pulled glass capillary needles to a 1/16" OD PEEK tubing?

19 October 2021 3,349 1 View

Internal standard for 5-hydroxymethyl Furfural from Fructose?

I'm trying to find a good internal standard for aqueous Fructose dehydration to 5-hydromethylfurfural, for use in an HPLC (due to the water). It doesnt appear any studies actually use an internal...

20 August 2021 9,623 1 View

Does LAMMPS provide support for Lubachevsky-Stillinger algorithm?

I am looking to produce maximal random packings using a Lubachevsky-Stillinger algorithm or variant. I don't see any provided fixes in LAMMPS for "growing" particles. I presume the alternative is...

11 July 2021 9,181 1 View

Why is energy not constant in an NVE VASP MD simulation?

I have a VASP MD simulation of a 2x2x1 supercell of Al2O3 totaling 120 atoms. The supercell was initially relaxed and then run for 1500 time steps (0.1 fs time step, 1e-7 EDIFF) in the NVE...

17 May 2021 3,598 2 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

THP-1 cells completely dying with PMA, help?

Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...

03 July 2024 3,884 5 View

What could be the expression profile of alkaline phosphatase from lactic acid bacteria?

I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...

02 July 2024 5,332 3 View

Can I do IPTG induction on OD600=2.6 in TB?

I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...

30 June 2024 6,502 5 View

How do you bleed HIV-infected humanized mice?

Hello everyone, I usually use the saphenous vein to collect blood from HIV-infected mice (for viral load, CD4/CD8, etc.). However, I would like to know what methods other researchers are using to...

27 June 2024 7,707 2 View

THP-1 differential markers (M0, M1 and M2). what do you recommend?

Hello, what THP-1 differential markers do you use in the Western-blot method? I am looking for proteins that are characteristic of M0, M1 and M2. Do you have something proven and reliable? Greetings

24 June 2024 9,025 0 View

Question about ELISA with the "Cattle IGF 1 (Insulin like growth factor) ELISA Kit" Cat: ELK5630?

Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...

13 June 2024 9,473 0 View

How to incease the yiald of recombinant protein expression ?

Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...

12 June 2024 9,616 8 View

Do you know a partner, friend, colleague, or someone else who was a Canadian gay (male) nurse who cared for PLWH during the HIV/AIDS pandemic?

My name is Carl GA Jacob. I am an Auxiliary Professor in the School of Nursing at the University of Ottawa. I am the author of the 2012 research titled: The Use of Experiential Learning in the...

10 June 2024 6,253 0 View

Emmanuel Ifeanyi Obeagu

Dear you can design the questionnaire and administer to the patient and get thier response in connection to employment.

Joyce P Yang

It might be useful to consult existing Stigma scales (e.g.:Article Measuring Stigma in People with HIV: Psychometric Assessment...

) and extract the items related to employment