How to calculate the orbital occupancy of Partial Density of States in VASP?

Hi, I have integrated the total DOS of a system up to the Fermi Energy and got the expected valence electrons. But when integrating the Partial DOS (PDOS) for each atom and summing these, the...

03 December 2019 2,714 3 View

Why my restiction enzyme treated chromasomal DNA has Plasmid results?

I conducted a practical whereby previously isolated plasmid and chromasomal DNAs that had been electrophoriesd in sepeerate gells where then both treated with the same restiction enzyme (EcoR1)...

11 September 2019 8,730 3 View

Where is my NTC contamination coming from?

I am currently performing qPCR and in the process of setting up relative standard curves to assess efficiency. I keep getting amplification in my NTC wells despite using new water and new SYBER...

13 August 2019 6,870 9 View

Intracellular dyes in patch pipette - cheap alternative to Alexa?

I generally include Alexa 488 hydrazide intracellular dye in my patch pipette to have a look at the morphology of the cell while I'm recording from it. It's getting a bit expensive now though,...

20 June 2019 1,464 3 View

Is there a published protocol for extracting lipids from skeletal muscle?

Hello, I am wanting to express mouse skeletal muscle contractile performance to lean tissue mass, and I had been advised in a piece of peer review for a paper that it is possible to perform such...

17 March 2019 716 1 View

Does anyone have any ideas for my research paper ? My topic is Steve Jobs ?

I need help on getting something to write about Steve Jobs. for my CIS term paper

17 September 2018 6,536 2 View

Whats the best way to extract and save venom from a live lionfish?

I'd like to pull venom from my pet lionfish. I can imagine that pressure and puncture of a basic seal on a beaker or something will work, but is there a good type of seal to use? and how should I...

21 March 2017 9,231 5 View

With the issue of seasonal tourism, what strategies can be put in place to combat this and spread tourism out through the year?

Seasonality at tourism destinations is a problematic issue as during the low months, resources aren't used and destinations cannot generate enough revenue. How can this be combatted in order to...

05 March 2017 1,631 3 View

Any advice on the vapor pressure behavior of Rose Bengal: outgassing or volatilization behavior?

I am using Rose Bengal as a photosensitizer coated on a substrate. To cure this layer we are considering use of a vacuum oven to remove the solvents but are concerned about outgassing of the RB....

08 October 2015 8,154 2 View

Where do I acquire zinc deficient soil?

Final Update: I've been in contact with the USDA & A&M's Ag Extension and they are in the process of combing through a recent soil survey to find soil that meets my specific needs. I ended...

30 November 2014 1,134 10 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

Why is ethics significant in business and how should one control unethical behavior ?

1. The significance of ethics to business and how to control bad behavior.

21 February 2021 882 14 View

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem?

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem.

21 February 2021 9,133 2 View

How to use bag of words for action prediction?

Hi everyone, I'm working on an action prediction (i.e., early recognition) using Bag-of-Words. I know how to use BOW, but regarding action prediction, there is a subtle problem. In the...

19 February 2021 1,442 4 View

Discuss the extent to which morality in African traditional societies can be classified under the Natural Law theory of morality?

How can one classified African traditional societies under natural law

18 February 2021 7,334 8 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

How to derive equations for a resonant buck converter with ZVS?

I am not understanding how each author is deriving the equations and the working principle. Individual authors are giving their own working principle explanation for different circuit topology....

12 February 2021 496 1 View