Where can I find these bacterial strains?

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What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

Beta galactosidase assay in milk?

I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...

12 June 2024 7,331 1 View

How to conduct/interpret power analysis for binary GLMM within-subjects with 2x3 independent factors?

Dear community, I can't seem to find any clear information on power analyses work so I'm throwing my question out onto the internet hoping for answers. :) Study Details I am doing a...

19 May 2024 9,214 4 View

Does TSO- salt in a 5% NaHCO3 solution turn the solution into a dark green color?

Here's the brief: 1. I synthesized alkyl gallates (ethyl gallate, butyl gallate, and amyl gallate) using Fischer esterification. 2. I used TsOH instead of concentrated H2SO4 as the catalyst. 3....

03 May 2024 6,737 5 View

Help on Modified Nanopore Sequencing to do Sanger-like sequencing for PCR amplicon from bisulfite converted DNA?

Hi, I'm planning to do sequencing using Nanopore. The main idea is to substitute Sanger Sequencing that usually used to sequence PCR amplicon from BCD (Bisulfite Converted DNA) using nanopore...

23 April 2024 6,637 0 View

Lyophilization of leaves, stem bark, fruits?

Is it recommended to freeze dry harvested leaves, stem bark, and fruits prior to trituration and extraction? I'll use the extracts for phytochemical analysis & in vitro bioassays. Thank you...

31 March 2024 5,920 4 View

Are nature-based solutions relevant to building a resilient society in the face of drinking water scarcity?

Although there have been a number of initiatives, the shortage of drinking water is still a problem for some communities. In this context, we can see that people's need for water is increasing as...

26 February 2024 5,285 3 View

How to remove DCU and its remaining traces from Steglich esterification?

Firstly, the compounds that I want to synthesize are alkyl gallates (ethyl gallate, butyl gallate, and hexyl gallate). The overall reaction: Gallic Acid + Alcohols + (THF, DCC, DMAP, 0 Celsius)...

25 February 2024 2,181 3 View

Has anyone worked with Science Paths - reportedly a book writing service?

Hello everyone, I receive many emails about different journals and publishers inviting manuscripts (some predatory, some not); however, received something new. Someone from Science Paths sent an...

12 December 2023 1,819 0 View

Achieving the SDGs by 2030: myth or reality?

According to a report by the Sustainable Development Solutions Network (SDSN) published in 2023, only 18% of SDG targets will be met by 2030. Should we expect a new global agenda?

26 November 2023 7,921 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View