Where can I find places that apply site-directed mutagenesis in Egypt ?

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Can biofloc system be applied in earthen ponds?

Dear colleagues Can I apply biofloc system in earthen ponds?

17 May 2024 2,685 0 View

High dsDNA concentration on Qubit but horrible, degraded gel electrophoresis?

Hello everyone! I am working on my undergraduate thesis. After I used the DNeasy® PowerSoil® Pro Kit to extract DNA from a coral mucus sample I use 2 microliters of DNA to measure the output's...

15 December 2023 4,751 1 View

Zinc oxide solution preparation?

For preparing a solution, the recipe mentions that Zinc acetylacetonate should be user. But at the moment we have zinc acetylacetonate+H2O. Can I use the latter instead. There is stirring step...

26 November 2023 303 0 View

Hello every one... Could you guid me to interpertate dielectric spectrogram and cole cole plot especially for the main variables or parameters ?

Dielectric permitivity spectrogram interpertation and cole cole plot especially for biological material

09 March 2023 5,691 0 View

Is there a commercially available kit for removing mouse proteins from serum or tissues?

I am attempting to detect a number of proteins expressed by a bacterium during infection in a mouse model. However, the presence of mouse proteins in the serum and tissues is hindering the results...

02 February 2023 2,629 1 View

Why is there in our time a decline in education and learning?

Has the development of technology led the human mind, especially the educated, to a lack of absorption and difficulty in learning despite the availability of technological means, unlike the...

27 October 2022 7,633 14 View

Surface plasmon resonance sensogram dotted and solid lines?

Hello, I received this SPR sensogram for small molecule binding affinity test on a protein. Would you please explain to me what are those dotted lined next to solid line? Thanks

19 May 2022 1,258 3 View

How can i dissolve Manganese (II) chloride in water?

I need to dissolve it in water for the preparation of SEM-TB media. I prepared 1M stock solution but it is cloudy not clear. I can not use it in the media preparation as it has to be clean.So what...

01 December 2021 3,545 5 View

Is there anyone who can give me some studies and statistics for brain drain from North Africa to Europe and North America?

01 November 2021 7,256 3 View

How to get my final stock solution??

I have a drug that comes in 10mg, how much would I add to obtain a final stock concentration of 100mM? The MW is 579.9and the drug is soluble in H2O at 52mg/ml. When I did the calculation I...

18 October 2021 2,745 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View