I need CEL1 enzyme to use it in my laboratory work to complete my PhD thesis. Unfortunately, I didn't find it anywhere. So I'm waiting for any request about companies name where is available or anyone who sell it for me.
thank you.
I have not seen it online but the excellent paper here
Plant Mol Biol Rep
DOI 10.1007/s11105-013-0606-z
details how to make it with just an ammonium sulphate precipitation. The paper also details the original purification from an earlier publication
Mojtaba Lotfi
Unfortunately I couldnt find any company to sell it online, which is a bit weird, but as you probably know, CEL I Endonuclease is naturally existed in Celery juice, which normally has been utilized for detection of heteroduplex and SNPs, specifically in confirmation of genome editing by CRISPR-Cas. In this link you will find a protocol which has been described an extraction method of CEL1 from Celery juice which could be done by your own.
https://brcf.medicine.umich.edu/cores/transgenic-animal-model/training-education/protocols/cel-i-assay-to-detect-non-homologous-endjoining-repair/
Also, based on your target in working with such a Endonuclease, you may be able to substitute it by T7E1 which is more common than CEL1 and could be purchased from NEB.
Good luck
Here are a lot of companies mentioned on this page. You may enquire in their sales dept: https://www.genecards.org/cgi-bin/carddisp.pl?gene=CEL
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