Hi everyone,
I am looking for a specific microbe and though someone here could help? For my research, I need a non-pathogenic bacterial host that is slightly halophilic. Ideally this host should be able to survive in fresh or seawater and preferably one who's genome has been sequenced in the past.
I am currently working with E. coli NEB 10-b and it is proving to be very sensitive to changes in ionic strength. I am also working with P. putida KT2440. Though I have managed to grow this host in LB media with an ionic strength of 0.5 M, they cannot survive the change to glucose minimal media at the same ionic strength (let alone surviving in seawater).
Does anyone have a suggestion or potential host in mind?
Thank you!
You might try with Vibrio strains, e.g., Vibrio sp. DSM14379 (renamed later as Vibrio ruber), see Danevcic et al. (2005) Biochim. Biophys. Acta. 1712:1-8. Vibrio harveyi might work as well (biosafety 1 level), and V. alginolyticus (however, a BSL2 organism). From my own experience, Vibrio grows well in LB.
You could use Vibrio Anguillarum strains. These are marine bacteria. Some isolates are fish pathogens, while others are not. A selection of virulent and avirulent isolates were sequenced (see Busschaert et al. 2014 at following link http://onlinelibrary.wiley.com/doi/10.1111/jfd.12290/abstract).
This is great, thank you both for your help. Vibrio does look like good potential hosts that are widely available on ATCC. Plus their optimal growth rates are typically around room temperature, is a plus for me. I am also considering some Shewanella strains as a potential host. I will verify the growth rate in freshwater of some of these strains and let you know the outcome.
Thanks again!
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