I am looking for a validated, reputable expression profile for miRNA in human liver cells. Thank you!
Rhys Gillman
You could try these links.
http://fantom.gsc.riken.jp/5/suppl/De_Rie_et_al_2017/vis_viewer_novel/#/human
http://guanlab.ccmb.med.umich.edu/mirmine/help.html
Best
Harsha
Good day!
Check out the following links:
http://zmf.umm.uni-heidelberg.de/apps/zmf/argonaute/organres.php
http://www.microrna.org/microrna/getExprTissueInfo.do
Hi everyone. I am trying to figure out how much of the Zn content in waste PCBs is likely to be ZnO. I know the majority of the Zn is alloyed or galvanised steel components. But I have found...
22 May 2023 5,026 0 View
For detection of phosphotyrosine level of a particular protein in a cell lysate, there seem to be two options: Immunoprecipitate the protein of interest, run on a gel and probe with a pan...
04 April 2023 5,840 4 View
-I am using Fura-2AM dye with probenecid to permiabilise TR146 oral epethelial cells. -flourescence is detected on a Flexstation. -Results from current experiments vary significaltly to previously...
29 March 2020 8,240 4 View
I need to identify the dissolved oxygen, moisture content, salinity, and pH level of soil samples over time. Is there some equipment or a probe that can do this? I keep coming across multimeters...
13 June 2018 9,802 4 View
I am currently conducting a study on 2 mileage tracking device of vehicles. One is the on-board odometer and the other is a digital tracker. The study is to determine if there is any significant...
07 March 2018 4,849 3 View
We are having issues with our conventional developer and are looking to either purchase a new machine or move to a digital method of visualization. we also need to be able to visualize lipid...
23 November 2017 5,533 0 View
I've inherited human blood samples from another lab and want to extract RNA from them. They're stored in Fastubes which it seems aren't manufactured anymore (I think were made by Endotell or...
30 March 2016 7,698 4 View
I need to find a material that fulfills these criteria: -Optical transparent -Resistant to IPA -Non NIR absorbing -Liquid before curing (so I can create custom parts) -Can hold shape, but deform...
04 March 2015 6,881 8 View
I have been trying to identify analogues to human growth factors in biological secretions. I am having trouble detecting EGF, even in the positive control. I have tried increasing the gel to a 20%...
27 August 2014 2,786 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...
18 July 2024 9,177 2 View
Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...
24 June 2024 3,507 2 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...
16 June 2024 9,723 2 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View
I'd like to receive notifications of call for papers for any journal pertaining to my expertise (immunology, rheumatology, immuno-mediated liver involvement) from researchgate. Thanks in advance...
01 June 2024 1,352 0 View
Reference for BMEL cells : Inducible differentiation and morphogenesis of bipotential liver cell lines from wild-type mouse embryos Hélène Strick-Marchand, Mary C Weiss doi:...
29 May 2024 7,198 0 View