Could a coating antibody also be used as detection antibody in an ELISA sandwich assay?

10 November 2015 1,371 7 View

What preservative should be used for Ag storage in an indirect ELISA?

I want to make an indirect ELISA (Ag+anti-Ag+anti-anti-agHRP). And I want to reconstitute the lyophilized antigen (peptides). The question is, what preservative should be used for Ag storage , if...

07 August 2015 9,676 3 View

What is the best method for obtaining derived-vaccine antigen to be used in in vitro T cell proliferation assays?

I want to make an assay of in vitro proliferation of PBMC in animals immunized using the same vaccine antigen. To do this I have to process the vaccine. Among the existing techniques as UV,...

09 October 2014 2,397 5 View

Does anyone know of any protocol to obtain an ethanolic extract of Euphorbia plants latex?

I want to make a study using the ethanolic extract of a Euphorbia latex on tumor cells in culture. I have tried some protocols, but do not get a good result, since the formation of precipitates in...

05 June 2014 1,136 3 View

What is the lifetime of a flow cytometer laser?

What is the lifetime of a flow cytometer laser?

02 March 2014 5,400 3 View

Is it possible to fix PBMCs and after one or two weeks add the antibodies (CD4/CD8) for immunophenotyping flow cytometry?

I need to urgently analyze some samples this week, but I have not yet antibodies. They take 2 or 3 weeks to arrive, so I want to know if I can keep the fixed cells. Will there be any change?

09 October 2013 4,276 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View