Could a coating antibody also be used as detection antibody in an ELISA sandwich assay?

10 November 2015 1,371 7 View

What preservative should be used for Ag storage in an indirect ELISA?

I want to make an indirect ELISA (Ag+anti-Ag+anti-anti-agHRP). And I want to reconstitute the lyophilized antigen (peptides). The question is, what preservative should be used for Ag storage , if...

07 August 2015 9,676 3 View

What is the best method for obtaining derived-vaccine antigen to be used in in vitro T cell proliferation assays?

I want to make an assay of in vitro proliferation of PBMC in animals immunized using the same vaccine antigen. To do this I have to process the vaccine. Among the existing techniques as UV,...

09 October 2014 2,397 5 View

Where can I buy LSCC-RP9 cell line?

07 August 2014 9,546 0 View

What is the lifetime of a flow cytometer laser?

What is the lifetime of a flow cytometer laser?

02 March 2014 5,400 3 View

Is it possible to fix PBMCs and after one or two weeks add the antibodies (CD4/CD8) for immunophenotyping flow cytometry?

I need to urgently analyze some samples this week, but I have not yet antibodies. They take 2 or 3 weeks to arrive, so I want to know if I can keep the fixed cells. Will there be any change?

09 October 2013 4,276 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View